Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Yeast extract | 0.500 |
| Dextrose | 10.000 |
| Calcium phosphate | 5.000 |
| Ammonium sulphate | 0.500 |
| Potassium chloride | 0.200 |
| Magnesium sulphate | 0.100 |
| Manganese sulphate | 0.0001 |
| Ferrous sulphate | 0.0001 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 16.3 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Pikovskaya's Broth is a modification of Pikovskayas agar medium originally modified by Sundara Rao and Sinha (1) for culturing phosphate solubilizing microorganisms. Both inorganic and organic phosphates exists in soil. Many naturally occurring soil fungi and bacteria are phosphate solubilizers and they play an important role in maintaining phosphorus balance of crop plants. This fact is exploited in culturing phosphate solubilzers which are able to solubilize bound phosphates. (2). Phosphate as calcium phosphate is present in the medium. Dextrose acts as energy source. Different salts and yeast extract supports the growth of organisms. The growth obtained in Pikovsyaayas broth (GM1719) may be detected for phosphate solubilization by subculturing or spot inoculation on Pikovskayas agar (M520). Phopshate solubilization is indicated as clearance around growth or colony.
Quality Control
Appearance
White to light yellow coloured granular medium.
Colour and Clarity of prepared medium
Whitish with flocculant precipitate opaque solution forms in tubes.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 48 hours (by spot inoculation on Pikovskaya's Agar).
| Organism | Growth | Phosphate solubilization* |
|---|---|---|
| #Aspergillus brasiliensis ATCC 16404 (00053*) | luxuriant | positive reaction, clear zone surrounding the colony |
| Bacillus subtilis ATCC 6633 (00003*) | good | Moderate clear zone surrounding the colony |
| Pencillium notatum ATCC 10108 | luxuriant | Positive reaction, clear zone surrounding the colony |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | Positive reaction, clear zone surrounding the colony |
Key:- # Formerly known as Aspergillus niger
Key:- * Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 10 -30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Pikovskaya’s Broth (Medium), Granulated |
|---|---|
| SKU | GM1719 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal Free (Microbial) |
| Packaging type | HDPE |
| References | 1.Sundara Rao W.V.B. and Sinha M.K., 1963, Ind. J., Agric. Sci., 33:272.2.N.S. Subba Rao, 1977, ‘Soil Microorganisms and Plant Growth’, Oxford and IBH Publishing Co., New Delhi. |
| Customized Product Available | No |
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