Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Meat peptone | 10.000 |
| Lactose | 10.000 |
| Sodium chloride | 5.000 |
| Bromo cresol purple | 0.010 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.01 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
DEV Lactose Peptone Broth is used for enrichment and titre determination of coliform bacteria.
Meat peptone provides carbon and other essential nutrients for growth. Sodium chloride is for the osmotic balance Lactose is the carbohydrate source. Bromocresol purple is a pH indicator which has a yellow colour below pH 5.3 and purple colour above pH 6.7. Due to the fermentation of lactose and the subsequent acid production the colour changes to yellow (1).
Quality Control
Appearance: Cream to yellow coloured granular medium
Colour and Clarity of prepared medium: Purple coloured clear solution
Reaction: Reaction of 2.5% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response: Cultural characteristics observed after an incubation at 37°C for 18-24 hours.
| Organism | Cultural Response |
|---|---|
| Enterobacter aerogenes ATCC 13048 | good |
| Escherichia coli ATCC 25922 | good |
| Klebsiella pneumoniae ATCC 13883 | good |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | DEV Lactose Peptone Broth, Granulated |
|---|---|
| SKU | GM1356 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.MacFaddin J.F., 1985, Media for Isolation - Cultivation - Identification - Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore. |
| Customized Product Available | No |
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