Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptic digest of animal tissue | 15.000 |
| Meat extract | 9.000 |
| Dipotassium hydrogen phosphate | 1.100 |
| Malachite green | 0.030 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.13 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Pseudomonas species is an environmental organism found in water and soil and on plants, including fruits and vegetables. Pseudomonas aeruginosa has the ability to survive in the aqueous environments like whirlpool bathwater, swimming pools etc (1). Whirlpools with elevated temperature, reduced chlorine and increased amounts of organic matter provide ideal conditions for the growth of P.aeruginosa. P.aeruginosa is commonly isolated from whirlpool waters that is coliform-negative(2).
Malachite Green Broth is recommended for the selective enrichment of P.aeruginosa as per Habs and Kirschner (3). It is also used for testing water samples as recommended by Schubert and Blum (4).
Meat extract and peptic digest of animal tissue serve as sources of essential nutrients required for bacterial metabolism. Dihydrogen potassium phosphate serves to buffer the medium. Malachite green makes the medium selective for P.aeruginosa while suppressing the growth of the accompanying flora. The medium can also be used as a single strength medium by suspending 8.4 g/litre of medium, depending upon the sample being tested.
Quality Control
Appearance
Light yellow to greenish yellow granulated media
Colour and Clarity of prepared medium
Peacock blue coloured clear solution without any precipitate in tubes
Reaction
Reaction of 2.51% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Cultural Response | ||
| Escherichia coli ATCC 25922 | >=10³ | inhibited |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Malachite Green Broth, Granulated |
|---|---|
| SKU | GM1266 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. ,, |
| Customized Product Available | No |
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