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Tartoff - Hobbs Broth, Granulated (Terrific Broth, Granulated)
As low as
₹8,950.00
Availability: In stock
Only %1 left
SKU:
GM1250
Synonym :
Terrific Broth
For the cultivation of recombinant strains of Escherichia coli.
Synonym :
Terrific Broth
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 12.000 |
| Yeast extract | 24.000 |
| Monopotassium phosphate | 2.200 |
| Dipotassium phosphate | 9.400 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.6 grams in 1000 ml distilled water containing 4 ml glycerol. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Tartoff Hobbs developed the Terrific Broth Medium for cultivation of recombinant Escherichia coli strains. These strains have extended growth phase when cultivated in this medium (1). Tartoff-Hobbs Medium supports high cellular density and mass and maintains the growth in the logarithmic phase for a long time. Due to this fact, it provides greater yields of recombinant proteins and plasmid DNA. Often, Tartoff-Hobbs (Terrific) Broth substitutes Luria Bertani Broth (GM1151/ M1151), to get enhanced yields of plasmid DNA and recombinant proteins. The procedures for inoculation, incubation and generation of recombinant strains are detailed by Sambrook et al (2).
Casein enzymic hydrolysate and yeast extract supply the necessary nutrients and cofactors for the excellent growth of recombinant strains of E. coli. The addition of extra peptone and yeast extract in the medium allows higher plasmid yield per volume. The two phosphates provide good buffering action to the medium. Glycerol is used as the carbohydrate source. Unlike glucose, glycerol is not fermented to acetic acid.
Quality Control
Appearance
Cream to light yellow coloured granular medium
Colour and Clarity of prepared medium
Light amber coloured clear solution without any precipitate.
Reaction
Reaction of 4.76% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours (Growth evident by turbidity).
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | good |
| Escherichia coli ATCC 39403 | 50-100 | good |
| Escherichia coli ATCC 47014 | 50-100 | good |
| Escherichia coli ATCC 53868 | 50-100 | good |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Synonym :
Terrific Broth
| Product Name | Tartoff - Hobbs Broth, Granulated (Terrific Broth, Granulated) |
|---|---|
| SKU | GM1250 |
| Synonym | Terrific Broth |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Tartoff K. D. and Hobbs C. A., 1987, Improved Media for Growing Plasmid and Cosmid Clones, Bethesda Res. Lab. Focus,9:12. |
| Customized Product Available | No |
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