Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the growth of Saccharomyces cerevisiae for use in molecular biology.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 20.000 |
| Yeast extract | 10.000 |
| Dextrose (Glucose) | 20.000 |
| Final pH (at 25°C) | 6.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 12.5 grams media. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Mix well and dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
YPD Broth (YEPD Broth) is recommended by Sherman (4) for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone-Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts (1). Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, the cells divide every 90 minutes (1). The medium composition aids in growth of Saccharomyces. Peptone provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support growth of S. cerevisiae.
Type of specimen
Isolated strain of Saccharomyces cerevisiae
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Gelatin capsule containing cream to yellow coloured granular media
Colour and Clarity of prepared medium
Light amber coloured clear solution in tubes.
Quantity
Each capsule contains 12.5 grams sufficient for 250 ml media.
Reaction
Reaction of 5.0% w/v aqueous solution at 25°C. pH: 6.5±0.2
pH
6.30-6.70
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | good-luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good-luxuriant |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers. #Formerly known as Aspergillus niger
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be contaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiEncap™ YPD Broth (HiEncap™ YEPD Broth) |
|---|---|
| SKU | EC1363CCL |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1. Sherman F., Meths. Enzymol. 194, 3 (1991). 2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. and Struhl K., 1994, Current protocols in molecular biology, Current Protocols, Brooklyn, N.Y |
| Customized Product Available | No |
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