HiMedia's 10X Wash Buffer is used to rinse microtiter plates during the coating process and between reagent addition steps of an ELISA at 1X concentration. The standardized formulation of this buffer effectively removes excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound assay components can be washed away without suppressing antigenantibody binding interactions, thereby reducing nonspecific background noise and increasing the specific signal.
10X Wash Buffer is used as a high performance washing solution for use in all ELISA formats. This wash Buffer is formulated with optimized pH stabilizers, salts and detergents which increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal. This buffer is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.