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Agar Agar, Type I
Agar Agar, Type I is recommended for preperation of culture media for microbiological analysis.
CAS Number :
9002-18-0
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Intended use
Agar Agar Type I is produced for use in bacteriological routine laboratory work, plant tissue culture media, pharmaceutical preparations, where clarity, compatibility are not of prime importance. When suspended in cold water, it swells but does not dissolve. However, it readily dissolves in boiling water and solubility is facilitated by soaking the powder in cold water.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Safety guidelines may be referred in individual safety data sheets.
Limitations
- It is biological origin product since variation in colour of powder and clarity may observed.
- Each lot of the product has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's requirement.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium prepared by the product.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature
Quality Control
- Appearance: Cream coloured powder homogeneous free flowing powder.
- Solubility: Freely soluble in hot water at temperatures above 85°C. Insoluble in cold water.
- Clarity: A firm solid, clear to slightly opalescent gel is formed at a concentration of 1.5% at 38-41 °C.
- pH: pH of 1.5% w/v aqueous solution at 25 °C 6.0 - 7.0
- Dye Diffusion: Agar dye diffusion :- 18-20mm
- Identification test: As per method specified in USP 2022
- A: Infrared absorption
- B: Iodine TS colours some of the fragments of Agar bluish black, with some areas reddish to violet.
- C: Agar forms a clear liquid, that congeals at 30-39°C to form a firm resilient gel, which does not liquefy below 80°C.
- Microbial Load :
- Bacterial Count : <= 1000 CFU/gram by plate method, when incubated at 30-35°C for not less than 3 days
- Yeast & mould Count : <= 100 CFU/gram by plate method, when incubated at 20-25°C for not less than 5 days.
- Test for pathogens : 1. Escherichia coli- Absent/gram of sample 2. Salmonella species- Absent/10 gram of sample 3. Pseudomonas aeruginosa- Absent/gram of sample 4. Staphylococcus aureus- Absent/gram of sample 5. Candida albicans- Absent/gram of sample 6. Clostridia- Absent/gram of sample
Tests
- Test for Water absorption : As per method specified in USP 2022 NMT 75 ml of water is absorbed by 5.0 g of agar
- Limit of Gelatin : As per method specified in USP 2022 No yellow precipitate is formed.
- Limit of Foreign Starch: As per method specified in USP 2022 The sample solution does not, upon cooling, produce a blue colour upon the addition of iodine TS.
- Growth Promotion Test : As per method specified in USP 2022
- Cultural response: Cultural response observed after an incubation at 35-37°C for 18-24 hours by preparing Nutrient Agar (M001) using Agar Agar, Type I as an ingredient.
Cultural Response
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 (WDCM00013) | Luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) | Luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923(WDCM 00034) | Luxuriant |
| Salmonella enterica subsp. enterica Typhi ATCC 6539 | Luxuriant |
| Streptococcus pyogenes ATCC 19615 | Luxuriant |
| Salmonella enterica subsp. enterica Enteritidis ATCC 13076 (WDCM 00030) | Luxuriant |
| Salmonella enterica subsp. enterica Typhimurium ATCC 14028 (WDCM00031) | Luxuriant |
| Yersinia enterocolitica subsp. enterocolitica ATCC 9610 (WDCM 00038) | Luxuriant |
| Yersinia enterocolitica subsp. enterocolitica ATCC 23715 (WDCM 00160) | Luxuriant |
Chemical Analysis :
- Gelling temperature : 38-41°C
- Melting Range: >=85°C
- Water (KF) : <=20%
- Total Nitrogen : <= 0.125%
- Arsenic (As): <= 3 ppm
- Lead(Pb) : <= 10 ppm
- Acid- Insoluble Ash (On dry-Weight basis) : <=0.5%
- Total Ash (On dry-weight basis) : <=6.5%
- Foreign organic matter : <=1.0%
- Limit of Foreign insoluble matter : <=15 mg in 7.5 gm of Agar
Storage and Shelf Life
Store below 30°C in tightly closed container and away from bright light. Use before expiry date on label. On opening, product should be properly stored in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques.
CAS Number :
9002-18-0
| Product Name | Agar Agar, Type I |
|---|---|
| SKU | GRM666 |
| CAS No | 9002-18-0 |
| EC No. | 232-658-1 |
| Customized Product Available | No |
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