Cell Biology

Pluronic® F-68 is a co-polymer of ethylene and propylene oxide. It is a non-ionic detergent and is used as an anti-foaming agent in cell culture. It helps in stabilizing the cell membranes against the shear forces exerted during batch culture processes. It is suitable for insect cell culture. TCL023 is sterile filtered 10% Pluronic® F-68 solution.

TCL035 is sterile filtered recombinant human insulin solution formulated to contain 10mg recombinant human insulin per ml.

Bovine albumin Fraction V, also known as Fraction V is a protein derived from the serum of a cow. Fraction V gets its name "Cohn Fraction V" on account of its fractionation by the Edward Cohn purification methodology using cold ethanol precipitation. It also serves as protective agent against toxic metabolites.TCL036 is sterile filtered 7.5% albumin bovine fraction V solution in Dulbecco's Phosphate Buffered saline.

HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued. TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

TCL077 is sterile solution formulated to contain 45% w/v glucose in tissue culture grade water. This solution is sterilized by autoclaving.

Giemsa stain is a mixture of methylene blue, eosin, and azure B. It is specific for the phosphate groups of DNA and attaches itself to where there are high amounts of adenine thymine bonding. Giemsa stain is mainly used for staining of peripheral blood smears and specimens obtained from the bone marrow. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations. TCL083 is a sterile filtered concentrated solution of Giemsa stain.

DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium is with 1gm per litre glucose, sodium bicarbonate and sodium pyruvate. It does not contain L-glutamine.

DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium contains L-glutamine, 1gm glucose per litre, sodium bicarbonate and sodium pyruvate.

DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium is with 1gm per litre of glucose, sodium bicarbonate, sodium pyruvate and L-glutamine. It does not contain folic acid.

SFF001P is intended for use in combination with Chemically Defined Serum Free CHO Medium (Product code: SFM007L / SFM00AP) and can be used in fed batch processes of industrial CHO cell lineages such as CHO-K1, CHO DHFR- and CHO-GS based clones.

SFM007AP is a animal component-free and chemically defined serum-free CHO medium devoid L-glutamine, sodium bicarbonate and phenol red. The medium is formulated with 0.1% Pluronic® F-68 to protect against mechanical shear damage.

Pluronic® F-68 is a co-polymer of ethylene and propylene oxide. It is a non-ionic detergent and is used as an anti-foaming agent in cell culture. It helps in stabilizing the cell membranes against the shear forces exerted during batch culture processes. It is suitable for insect cell culture. TCL023 is sterile filtered 10% Pluronic® F-68 solution.

TCL035 is sterile filtered recombinant human insulin solution formulated to contain 10mg recombinant human insulin per ml.

Bovine albumin Fraction V, also known as Fraction V is a protein derived from the serum of a cow. Fraction V gets its name "Cohn Fraction V" on account of its fractionation by the Edward Cohn purification methodology using cold ethanol precipitation. It also serves as protective agent against toxic metabolites.TCL036 is sterile filtered 7.5% albumin bovine fraction V solution in Dulbecco's Phosphate Buffered saline.

ITS supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

ITS-E supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

ITS-P supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

ITS-B supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued. TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Glasgow's Minimum Essential Medium is a modification of Basal Medium Eagle (BME). AT058 is Glasgow's Minimum Essential Medium with L-glutamine and non-essential amino acids. It does not contain Sodium phosphate and tryptose phosphate broth.

Glasgow's Minimum Essential Medium is a modification of Basal medium Eagle (BME). AT069 is Glasgow's Minimum Essential Medium with L-glutamine and tryptose phosphate broth.

Glasgow's Minimum Essential Medium is a modification of Basal medium Eagle (BME). AT102 is Glasgow's Minimum Essential Medium with L-glutamine. It does not contain tryptose phosphate broth.

Chicken embryo is a widely used medium for the initial isolation and propagation of viruses and the production of vaccines. Chick Embryo Fibroblast Medium is a proprietary formulation specially developed to culture chicken embryo fibroblast cells. AT168 is Chick Embryo Fibroblast Medium with LGlutamine.

Tryptose phosphate broth is used to supplement cell culture media. It is most commonly used to enhance the growth supporting property of Glasgow’s Minimum Essential Medium for BHK-21 cells. It is also used in insect cell culture media.

Meets USP-NF, EP, BP, JP and IP testing specifications

β-Propiolactone is an organic compound of the lactone family. It is commonly used to sterilize vaccines, enzymes, tissue grafts, nutrient broths, blood plasma, water, milk, surgical instruments. Its sporicidal action is used against vegetative bacteria,pathologic fungi and viruses. It has been used as an intermediate in the production of acrylic acid and esters. It has also been used to inactivate viruses for use in vaccines for animals and human.  β-propiolactone may be used as a replacement for formaldehyde in gaseous decontamination.It does not condense as a non-volatile residue and thus the time required to aerate an enclosure after application of the disinfectant is considerably less than that for formaldehyde. One more advantage of β-propiolactone over other disinfectants is active even at relatively low temperatures.

HiViral™ Transport medium is a specially formulated medium for collection, transport and long term freeze storage of viruses. It is designed to maintain the optimum viability and virulence of the viral sample.

Human serum particularly finds application for human cell culture, tissue engineering, transplantation and cell therapy applications. RM10986 is human serum which is off-the-clot serum, processed from pooled human AB group blood that has had natural coagulation.

Fetal bovine serum is collected from approved abattoirs in USA. US Origin FBS is considered the world’s standard for quality and is one of the most widely used serum in the world.

RM10951 is heat inactivated and gamma irradiated Fetal bovine serum. Heat inactivation is done to destroy heat labile components such as complement that can lead to complement mediated cell lysis. Complement proteins, antibodies and enzymes present in the serum are inactivated by heat inactivation. It is gamma irradiated with 25- 35kGy dose. The serum is collected from approved abbatoirs in USA. US Origin FBS is considered the world’s standard for quality and is one of the most widely used serum in the world.

RM10969 is Fetal bovine serum collected from approved abattoirs in New Zealand. New Zealand is considered to be free of BSE and FMD and belongs to GBR 1 category and thus, assures the lowest possible viral risk. New Zealand-origin serum is most commonly used in the production of many human and animal biopharmaceuticals and is proven to support the needs of industrial biopharmaceutical manufacturers.

RM10978 is gamma irradiated Fetal Bovine Serum. Gamma irradiation minimizes the risk of viruses in animal origin material as it reduces or eliminates wide range of viruses and mycoplasmas, which may be present in the serum after filtration, thus enhancing biological safety of the serum. It is gamma irradiated with 25- 35kGy dose. This serum has been collected from approved abattoirs in New Zealand. New Zealand is considered to be free of BSE and FMD and belongs to GBR 1 category and thus, assures the lowest possible viral risk.

RM9951 is fetal bovine serum collected from AQIS (Australian Quarantine and Inspection Service) approved abattoirs in Australia. Australia is considered to be free of BSE and FMD and belongs to GBR 1 category. Australian origin serum is most commonly used in the production of many human and animal biopharmaceuticals and is proven to support the needs of industrial biopharmaceutical manufacturers.

New Born Calf Serum is processed from calves that are 3-10 days old. It contains higher levels of proteins and immunoglobulins and less growth factors than Fetal Bovine Serum (FBS). It is a cost effective alternative to FBS and can be used for culturing most mammalian cell lines. It has proved to be an efficient growth promoter in 3T3 cells (mouse fibroblasts).

New Born Calf Serum is processed from calves that are 3-10 days old. It contains higher levels of proteins and immunoglobulins and less growth factors than Fetal Bovine Serum (FBS). It is a cost effective alternative to FBS and can be used for culturing most mammalian cell lines. It has proved to be an efficient growth promoter in 3T3 cells (mouse fibroblasts).

Pig serum is collected from animals younger than 1 year old. The constituents of human blood and porcine serum are similar, hence porcine serum is found to be very useful in lymphocyte culture work as well as in chromosome banding studies involving lymphocyte culturing.