HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended for cultivation of phytopathological fungi and other fungi
Composition
| Ingredients | g/L |
|---|---|
| Corn meal, infusion from | 50.000 |
| Dextrose (Glucose) | 2.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Corn Meal Dextrose Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Corn Meal Agar is a general purpose medium used for the cultivation of fungi and for the study of Candida species for the chlamydospore production. Corn Meal Agar with Dextrose is used for the cultivation of commonly occuring as well as phytopathological fungi. The addition of dextrose enhances the chromogenesis of some species of Trichophyton (1). Pollack and Benham (2) have described the usefulness of this medium for studying the morphology of Candida. This is a very simple formulation containing corn meal infusion, dextrose and agar. However this infusion has enough nutrients to enhance the growth of fungi. Addition of dextrose to the medium supports more luxuriant growth of some fungi as compared to the medium without dextrose, but dextrose supplemented Corn Meal Agar should not be used for chlamydospores production, some Candida species lose their ability of chlamydospore formation by repeated sub culturing.
Type of specimen
Plant samples
Specimen Collection and Handling
For plant samples follow appropriate techniques for handling specimens as per established guidelines (2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- A non-selective and selective medium should be inoculated for isolation of fungi from conceivably contaminated specimens.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile glass bottle containing slightly opalescent Corn Meal Dextrose Agar
Colour
Light amber coloured medium
Quantity of medium
500 ml of medium in glass bottle.
pH
5.80-6.20
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 23-27°C for upto 4 days. (For observing Chlamydospore formation: Using a straight wire, make a deep cut in the Corn Meal Agar plate with inoculum. Place a flamed sterile coverslip over the line of inoculum. After incubation, the streaks are examined microscopically, through the coverslip,using low and high power objectives, for chlamydospore formation.)
| Organism | Inoculum (CFU) | Growth | Chlamydospores | Recovery |
|---|---|---|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | negative | |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | positive | >=70% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | negative | >=70% |
| Saccharomyces uvarum ATCC 28098 | 50-100 | luxuriant | negative | >=70% |
Key: * Corresponding WDCM numbers. # - Formerly known as Aspergillus niger
Storage and Shelf Life
On receipt store between 15-30°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Corn Meal Dextrose Agar |
|---|---|
| SKU | SM150D |
| Customized Product Available | No |
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