HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Sabouraud Dextrose Agar Slant is used for the cultivation of yeasts, moulds and aciduric bacteria from clinical specimen and non clinical samples.
Composition
| Ingredients | g / L |
|---|---|
| Dextrose (Glucose) | 40.000 |
| Mycological, peptone | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Streak the test inoculum aseptically into the slant and incubate at appropriate conditions.
Principle And Interpretation
Sabouraud Dextrose Agar is Carlier's modification (1) of the formulation described by is a modification of Sabouraud Dextrose Agar which is described by Sabouraud (2) for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections. This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (3).Mycological peptone provides nitrogenous compounds. Dextrose provides an energy source. High dextrose concentration and low pH favors fungal growth and inhibits contaminating bacteria from test samples (4).
Type of specimen
Clinical samples: skin scrapings, Food samples; Cosmetics.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines(5,6,7).
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (8,9).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
- Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical conditions.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate
- the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical tests should be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Sabouraud Dextrose Agar slant in glass tube.
Colour of medium
Light amber coloured medium
Quantity of medium
8ml of medium in glass tube
pH
5.40-5.80
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 20-25°C for 48-72 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | Luxuriant (white colonies) |
| Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Lactobacillus casei ATCC 334 | 50-100 | luxuriant |
| Trichophyton rubrum ATCC 28191 | - | luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | Sabouraud Dextrose Agar Slant |
|---|---|
| SKU | SL036 |
| Customized Product Available | No |
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