HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Streptococcus lactis Differential HiVeg™ Agar Base
For differentiation of citrate utilizing lactic Streptococci-Lactococcus lactis (Streptococcus lactis) subspecies diacetylactis from citrate nonutilizing Lactococcus lactis (Streptococcus lactis) and Lactococcus lactis (Streptococcus lactis) subspecies cremoris.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| Nonfat (Skim) Milk | 10.0 |
| HiVeg hydrolysate No. 3 | 2.5 |
| Dextrose | 5.0 |
| Agar | 15.0 |
Final pH (at 25°C) 6.6 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 32.5 grams in 1000 ml distilled water. Heat to boiling with stirring to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 12 minutes. Cool to 45°C and aseptically add (30 minutes steam sterilized solutions) 10 ml of 10% potassium ferricyanide and 10 ml of citrate solution containing 0.25 g ferric citrate and 0.25 g sodium citrate. Gently mix and pour into the sterile petri plates. Dry the plates in dark for 24 hours at 30°C.
Principle and Interpretation
This medium is prepared by completely replacing animal based peptones with vegetale based peptones thus making the medium BSE/TSE risk free. Streptococcus Lactis Differential HiVeg Agar Base is the modification of Streptococcus Lactis Differential Agar Base which is formulated as described by Kempler and McKay (1) and recommended by APHA (2) for differentiation of citrate utilizing lactic Streptococci - Lactococcus lactis (Streptococcus lactis) subspecies diacetylactis from citrate non-utilizing Lactococcus lactis (Streptococcus lactis) and Lactococcus lactis (Streptococcus lactis) subspecies cremoris.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Product Profile
| Vegetable based (Code MV)® | Animal based (Code M) |
|---|---|
| MV925 | M925 |
| HiVeg hydrolysate No. 3 | Peptonised milk |
| Recommended for | Differentiation of citrate utilizing lactic Streptococci-Lactococcus lactis (Streptococcus lactis) subspecies diacetylactis from citrate non-utilizing Lactococcus lactis (Streptococcus lactis) and Lactococcus lactis (Streptococcus lactis) subspecies cremoris. |
|---|---|
| Reconstitution | 32.5 g/l |
| Quantity on preparation (500g) | 15.38 L |
| pH (25°C) | 6.6 ± 0.2 |
| Supplement | 10% Potassium ferricyanide and citrate solution, |
| Sterilization | 115°C / 12 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Colour and Clarity
Light yellow coloured, opaque gel with white precipitate forms in petri plates.
Reaction
Reaction of 3.25% w/v aqueous solution is pH 6.6 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 18-48 hours with added 10% Potassium ferricyanide and citrate solution.
| Organisms (ATCC) | Growth |
|---|---|
| Streptococcus cremoris (19257) | good-luxuriant |
| Streptococcus lactis (8000) | good-luxuriant |
| Streptococcus lactis subsp. diacetylactis | good-luxuriant |
| Product Name | Streptococcus lactis Differential HiVeg™ Agar Base |
|---|---|
| SKU | MV925 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Kempler G. M. and McKay L. L., 1980, Appl. Environ. Microbiol., 39:926. |
| Customized Product Available | No |
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