HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
B. C. Motility Test HiVeg Medium is used for cultivation and examination of motility of Bacillus cereus strains.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 10.0 |
| Yeast extract | 2.5 |
| Dextrose | 5.0 |
| Disodium phosphate | 2.5 |
| Agar | 3.0 |
Final pH (at 25°C) 7.4 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 23 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in 2-3 ml amounts in screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubes to cool in an upright position.
Principle and Interpretation
B. C. Motility Test HiVeg Medium is prepared by replacing Casein enzymic hydrolysate with HiVeg hydrolysate which is free of BSE/TSE risks. This medium is modification of B.C. Motility Test Medium formulated as per APHA (1) for examination of motility of Bacillus cereus. The medium contains HiVeg hydrolysate, yeast extract and dextrose that provide nutrients. Disodium phosphate helps in maintaining the pH. Agar content of the medium is crucial for determining motility. 0.3% agar renders medium semisolid in which motile bacteria produce diffused turbidity due to growth, while nonmotile bacteria exhibit a line of growth along the line of inoculation. This medium is inoculated by stabbing down the center with 3 mm loopful of culture and incubated at 18 - 24 hours at 30°C. Rhizoid strains of Bacillus cereus var mycoides produce characteristic fuzzy growth due to expansion of the filamentous growth but they are not motile by means of flagella.
Quality Control
Appearance of powder: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Product Profile
Vegetable based (Code MV)
| MV906 |
| HiVeg hydrolysate |
Animal based (Code M)
| M906 |
| Casein enzymic hydrolysate |
Recommended for
Cultivation and examination of motility of Bacillus cereus
Reconstitution
23.0 g/l
Quantity on preparation (500g)
21.73 L
pH (25°C)
7.4 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Gelling
Semisolid, comparable with 0.3% Agar gel.
Colour and Clarity
Yellow coloured, clear to slightly opalescent gel forms in tubes as butts.
Reaction
Reaction of 2.3% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 18 - 24 hours.
| Organisms (ATCC) | Growth | Motility |
|---|---|---|
| Bacillus anthracis (14578) | luxuriant | - |
| Bacillus cereus (10876) | luxuriant | + |
| Bacillus cereus var mycoides | luxuriant | - |
| Bacillus thuringiensis (10792) | luxuriant | + |
Key: + = Positive reaction, growth away from stab inoculation - = Negative reaction, growth along the stab inoculation
| Product Name | B.C. Motility Test HiVeg™ Medium |
|---|---|
| SKU | MV906 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Mortimer P.R. and McCann.G, 1974, Lancet, 104:3. |
| Customized Product Available | No |
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