HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended for cultivation and enumeration of Lactobacilli and other acidophilic bacteria.
Composition
| Ingredients | g/L |
|---|---|
| Tomato juice (400 ml) | 20.000 |
| HiVeg™ peptone | 10.000 |
| HiVeg™ hydrolysate No. 3 | 10.000 |
| Agar | 20.000 |
| Final pH (at 25°C) | 5.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 60.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Lactic acid bacteria are acid-tolerant, non-sporulating rods or cocci widely distributed in nature and historically linked to food fermentation. Lactobacilli form the normal flora of the human mouth, intestinal tract and vagina and may therefore be recovered from pathological specimens as contaminants (1). Tomato juice was included in media for lactobacilli (2) and was found to be advantageous for its growth, particularly Lactobacillus acidophilus (3).
Tomato Juice Agar, Special is formulated as per Jay (4,5) for the direct plate count of lactobacilli and other acidophilic bacteria, especially from saliva (6). Tomato Juice Agar, Special is similar to Tomato Juice Agar, except that the agar concentration is increased to 20 grams per liter and the pH is adjusted to 5.0 in the former. Tomato Juice HiVeg™ Agar, Special is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. Tomato juice provides an acid environment and is also a source of carbon, and other essential nutrients. HiVeg™ hydrolysate No. 3 provides lactose, which acts as the energy source. HiVeg™ peptone provides nitrogenous, carbonaceous compounds, trace elements and other essential growth nutrients. The low pH of medium inhibits many commensal bacteria and encourages growth of Lactobacilli. Tomato Juice Agar, Special is more selective than Tomato Juice Agar (6).
Type of specimen
Please add specimen
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium
Medium amber coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 6.0% w/v aqueous solution at 25°C. pH: 5.0±0.2
pH
4.80-5.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Lactobacillus acidophilus ATCC 4356 (00098*) | 50-100 | luxuriant | >=50% |
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant | >=50% |
| Lactobacillus leichmannii ATCC 4797 | 50-100 | luxuriant | >=50% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Tomato Juice HiVeg™ Agar, Special |
|---|---|
| SKU | MV879 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.2.Mickle F. L. and Breed R. S.,1925, Technical Bulletin 110, N.Y. State Agriculture Exp. Station, Geneva, N.Y.3.Kulp W. L., 1927, Science 66:512. |
| Customized Product Available | No |
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