HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Rippey-Cabelli HiVeg™ Agar Base
Used for differential and selective isolation of Aeromonas hydrophila species from water samples using membrane filtration technique.
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Intended Use
Rippey-Cabelli HiVeg Agar Base is recommended for differential and selective cultivation of Aeromonas hydrophila from water samples using membrane filter technique.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate No. 1 | 5.0 |
| Trehalose | 5.0 |
| Yeast extract | 2.0 |
| Sodium chloride | 3.0 |
| Potassium chloride | 2.0 |
| Magnesium sulphate | 0.2 |
| Iron (III) Chloride | 0.1 |
| Bromo thymol blue | 0.04 |
| Agar | 15.0 |
| Final pH (at 25°C) | 8.0 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | ||
|---|---|---|---|
| MV859 | HiVeg hydrolysate No. 1 | M859 | Tryptose |
| Recommended for | Differential and selective cultivation of Aeromonas species from water samples using membrane filter technique. |
| Reconstitution | 32.34 g/l |
| Quantity on preparation (100g) | 3.09 L |
| pH (25°C) | 8.0 ± 0.2 |
| Supplement | Rippey-Cabelli Supplement (FD107), Ethanol. |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2-8°C. |
Directions
Suspend 16.17 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 10 ml ethanol and rehydrated contents of 1 vial of Rippey-Cabelli Supplement (FD107). Mix well before pouring into sterile petriplates.
Principle and Interpretation
This medium is prepared by replacing Tryptose with HiVeg hydrolysate No 1 which makes the medium free of BSE/ TSE risks. Rippey-Cabelli HiVeg Agar Base is the modification of Rippey-Cabelli (RC) Agar formulated by Rippey and Cabelli (1). The medium is differential as it depends on ability of organisms to ferment trehalose. HiVeg hydrolysate and yeast extract support the growth of Aeromonas species. Ampicillin, and ethanol are the selective agents inhibiting gram positive bacteria, coliforms, Shigella species, Proteus mirabilis and Actinomyces. Ethanol inhibits overgrowth of Klebsiella species on the filter. Ampicillin is unsuitable as a selective agent with Plesiomonas (2). The limitation of medium is that it fails to recognize lysine-positive Aeromonas strains. As most enteric organisms ferment trehalose, specificity of this medium is insufficient due to clear characteristics to distinguish Aeromonas colonies from the Enterobacteriaceae (3). Sensitivity and specificity are higher when pure cultures are used (4).
Quality Control
Appearance of powder: Light green coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Dark green coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 3.23% w/v aqueous solution is pH 8.0 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24 hours on addition of Rippey-Cabelli Suplement (FD107).
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Trehalose fermentation |
|---|---|---|---|---|
| Aeromonas hydrophila (7966) | 102-103 | luxuriant | >50% | + |
| Escherichia coli (25922) | 102-103 | none-poor | <20% | - |
| Shigella flexneri (12022) | 102-103 | inhibited | 0% | - |
| Staphylococcus aureus (25923) | 102-103 | inhibited | 0% | - |
Key: + = acid production, yellow colour
- = no colour change (blue-green colour)
References
- Rippey S.R. and Cabelli V.J., 1979, Appl. Environ. Microbiol. 38 (1):108.
- Von Graevenitz A. and Bucher C., 1983, J. Clin. Microbiol, 17 (1):16.
- Roland, F.P., 1977, Med. Microbiol. Immunol., 163:241.
- MacFaddin J.F., 1985, Media for Isolation Identification Cultivation and Maintenance of Medical bacteria, Vol. I Williams and Wilkins, Baltimore.
| Product Name | Rippey-Cabelli HiVeg™ Agar Base |
|---|---|
| SKU | MV859 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Eaton A. D., Clesceri L. S. and Greenberg A. E., (Eds.), 1995, Standard Methods for the Examination of Water andWastewater, 19th Ed., American Public Health Association, Washington, D.C. |
| Customized Product Available | No |
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