HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended as a non-selective differential medium for the detection of enteric pathogens.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone | 7.000 |
| Sodium chloride | 5.000 |
| Lactose | 15.000 |
| Litmus | 1.200 |
| Agar | 13.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 41.2 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Drigalski Litmus Lactose Agar is formulated as per Drigalski and Conrad (1) as a differential medium for the detection of enteric pathogens from water, meat, milk and other food materials. The medium contains lactose as the source of carbon and fermentable carbohydrate. Drigalski Litmus Lactose HiVeg™ Agar is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ peptone supplies essential nitrogenous nutrients to the microorganisms. Litmus is the pH indicator in the medium. Lactose fermenters produce acid and thus change the colour of litmus to red forming red, colonies. Lactose non-fermenters form blue colonies on the medium. Inoculate culture from primary fermentation tubes showing gas either by four-quadrant streaking on the medium or by serial dilution and pour plate technique (2).
Type of specimen
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Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder, may have slight dye particles
Gelling: Firm, comparable with 1.3% Agar gel.
Colour and Clarity of prepared medium: Purplish blue coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.12% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH: 7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | fair-good | 40-50% | orange-red |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% | orange- red |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=70% | blue |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | luxuriant | >=70% | blue |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good | 50-70% | orange- red |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Drigalski Litmus Lactose HiVeg™ Agar |
|---|---|
| SKU | MV659 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Drigalski V. and Conrad H., 1902, Z. Hyg. Infektionskr., 39:283. 2.MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Volume I, Williamsand Wilkins, Baltimore. |
| Customized Product Available | No |
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