HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 15.00 |
| Papaic digest of soyabean meal | 5.00 |
| Sodium chloride | 10.00 |
| Agar | 15.00 |
Final pH (at 25°C) 7.3 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV593 | M593 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Product Data
| Recommended for | Use when 1% sodium chloride is needed in the medium. |
|---|---|
| Reconstitution | 45.0 g/l |
| Quantity on preparation (500g) | 11.11 L |
| pH (25°C) | 7.3 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Directions
Suspend 45.0 grams in 1000 ml distilled water. Heat to boiling to dissolve completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate which is free from BSE/TSE risks associated with animal based peptones. Tryptone Soya HiVeg Agar with added sodium chloride is the modification of Tryptone Soya Agar with added sodium chloride which is a nutritious general purpose medium recommended for use when 1% Sodium chloride is needed in medium. HiVeg hydrolysate and Papaic digest of soyabean meal provide nitrogenous compounds and other growth factors. The medium contains slightly high concentration of sodium chloride which provides the ions and also maintains osmotic equilibrium.
Quality Control
Appearance: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour & Clarity: Light amber coloured clear to slightly opelsent gel forms in petri plates.
Reaction: Reaction of 4.5% w/v aqueous solution is pH 7.3 ± 0.2 at 25°C
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Neisseria gonorrhoeae (19424) | 102-103 | luxuriant | >70% |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | >70% |
| Staphylococcus epidermdis (12228) | 102-103 | luxuriant | >70% |
| Streptococcus pyogenes (19615) | 102-103 | luxuriant | >70% |
| Product Name | Tryptone Soya HiVeg™ Agar w/ added NaCl |
|---|---|
| SKU | MV593 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| Customized Product Available | No |
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