HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg® peptone | 5.0 |
| HiVeg® extract | 3.0 |
| Lactose | 10.0 |
| Sodium chloride | 5.0 |
| China blue | 0.3 |
| Agar | 15.0 |
Final pH (at 25°C) 7.0 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | ||
|---|---|---|---|
| MV580 | M580 | ||
| HiVeg® peptone | Peptic digest of animal tissue | ||
| HiVeg® extract | Beef extract | ||
| Recommended for | Differentiation and enumeration of bacteria in milk. | ||
| Reconstitution | 38.3 g/l | ||
| Quantity on preparation (500g) | 13.05 L | ||
| pH (25°C) | 7.0 ± 0.2 | ||
| Supplement | None | ||
| Sterilization | 121°C / 15 minutes. | ||
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. | ||
Directions
Suspend 38.3 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
The medium is prepared by using HiVeg® peptone and HiVeg® extract which are free from BSE/TSE risks. China Blue Lactose HiVeg® Agar is the modification of China Blue Lactose Agar, which was first designed by Brandl and Sobeck-Skal (1). It is a standard, non-inhibitory medium used for the differentiation and enumeration of bacteria in milk. China blue is a pH indicator which helps in differentiating lactose fermenters and lactose non-fermenters. However, this medium does not restrict the growth of cocci and hence can be used for the detection of Streptococci and Staphylococci as well. China Blue Lactose HiVeg® Agar plates can be either streak inoculated directly or used for the pour-plate technique. Decimally diluted milk can be added to the molten, cooled medium.
Quality Control
Appearance of powder
Greenish yellow coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Light blue coloured, slightly opalescent gel forms in petri plates.
Reaction
Reaction of 3.83% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 22 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis (29212) | 102-103 | luxuriant | >70% | blue |
| Escherichia coli (25922) | 102-103 | luxuriant | >70% | blue |
| Proteus vulgaris (13315) | 102-103 | luxuriant | >70% | colourless |
| Salmonella serotype Typhi (6539) | 102-103 | luxuriant | >70% | colourless |
| Shigella flexneri (12022) | 102-103 | luxuriant | >70% | colourless |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | >70% | colourless |
MV580 China Blue Lactose HiVeg® Agar
- Control
- Escherichia coli
- Enterococcus faecalis
- Staphylococcus aureus
| Product Name | China Blue Lactose HiVeg® Agar |
|---|---|
| SKU | MV580 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Thomas S. B., 1974, the Microflora of Bulk Collected Milk- Part 2, Dairy Ind. Int. 39 (8): 279 2.De Vris T. 1975, Neth. Milk Dairy J., 29:127 3.Cousin M. A., 1982, J. Food Prot., 45:172 4.Brandl E. and Sobeck - Skal E., 1963, Milchwiss. Ber., 13:1. |
| Customized Product Available | No |
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