HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Differential Reinforced Clostridial HiVeg Broth is used for the cultivation of Clostridia from water.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.0 |
| HiVeg extract | 10.0 |
| Yeast extract | 1.5 |
| Starch | 1.0 |
| Sodium acetate, hydrated | 5.0 |
| Glucose | 1.0 |
| L-Cysteine hydrochloride | 0.5 |
| Final pH (at 25°C) | 7.2 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Technical Details
Product Profile
| Type | Code | Details |
|---|---|---|
| Vegetable based | MV549 | |
| HiVeg peptone | ||
| HiVeg extract | ||
| Animal based | M549 | |
| Peptic digest of animal tissue | ||
| Beef extract |
| Recommended for | Cultivation of Clostridia from water |
| Reconstitution | 29.0 g/l |
| Quantity on preparation (500g) | 17.24 L |
| Quantity on preparation (100g) | 34.48 L |
| pH (25°C) | 7.2 ± 0.2 |
| Supplement | Sodium sulphite, Ferric citrate |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Directions
Suspend 29 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just before use add 0.5 ml filter sterilized solution prepared by mixing equal volumes of 4% w/v solution of Sodium sulphite and 7% w/v Ferric citrate to 25 ml of single strength medium or 0.4 ml and 2 ml to 10 ml and 50 ml of double strength medium respectively. Mix well.
Principle and Interpretation
This medium is prepared by using HiVeg peptone and HiVeg extract which is free from BSE/TSE risks. Differential Reinforced Clostridial HiVeg Broth is the modification of Differential Reinforced Clostridial Medium which is based on the formulation described by Barnes and Ingram (1) and
Gibbs and Freame (2) for the sulphite reducing Clostridia from food and for enumeration in water by multiple tube method. Differentiation is based on the ability to reduce sulphite to sulphide to form iron sulphide resulting in black colour. HiVeg peptone, HiVeg extract, yeast extract, starch, sodium acetate provide essential nutrients for bacterial metabolism. Glucose is the source of energy. L-Cysteine hydrochloride acts as reducing agent.
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greensh tinge, homogeneous, free flowing powder.
Colour and Clarity: Light yellow coloured, clear solution without any precipitate.
Reaction: Reaction of 2.9% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 1 week with added 4% w/v solution of Sodium sulphite and 7% w/v Ferric citrate.
| Organisms (ATCC) | Growth | H₂S production |
|---|---|---|
| Clostridium perfringens (13124) | good - luxuriant | + |
| Clostridium sporogenes (11437) | good - luxuriant | + |
Key : + = blackening of medium
References
- Barnes E.M. and Ingram M., 1956, J. Appl. Bacteriol., 19(1):117.
- Gibbs B.M. and Freame B., 1965, J. Appl. Bacteriol., 28(1):95.
| Product Name | Differential Reinforced Clostridial HiVeg™ Broth Base |
|---|---|
| SKU | MV549 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Hirsch A. and Grinstead E., 1954, J. Dairy Res. 21:101 2.Barnes E. M. and Ingram M., 1956, J. Appl. Bacteriol., 19(1):117. 3.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone |
| Customized Product Available | No |
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