HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Modified CPLM HiVeg™ Medium Base (Trichomonas Modified CPLM HiVeg™ Medium Base)
Recommended for cultivation of Trichomonas species with addition of horse serum and antibiotics.
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Intended Use
Recommended for cultivation of Trichomonas species with addition of horse serum and antibiotics.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone | 32.000 |
| HiVeg™ extract No. 2 | 20.000 |
| Maltose | 1.600 |
| L-Cystine hydrochloride | 2.400 |
| Final pH (at 25°C) | 6.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 56.0 grams in 900 ml Ringer's solution (4th strength). Heat if necessary to dissolve the medium completely. Distribute in bottles in 90 ml amounts and sterilize by autoclaving at A115°C for 10 minutes. Cool to 45-50°C and add aseptically following (per 90 ml of medium).
- Sterile inactivated Horse Serum 10 ml
- Sterile Penicillin Streptomycin Solution 1 ml
- Sterile Nystatin Solution 1 ml
Mix thoroughly and distribute in suitable aliquots with sterile precautions.
Penicillin Streptomycin solution
| Penicillin | 1 x 1,00,000 units |
| Streptomycin Sterile | 0.1 g |
| distilled water | 10 ml |
Nystatin Solution
| Nystatin Solution | 5 x 10,000 units |
| Sterile distilled water | 10 ml |
The addition of antibiotics is not necessary for routine subcultures but is essential for clinical diagnostic cultures and for isolating axenic cultures
Principle And Interpretation
Trichomonas is a protozoan, similar to bacteria. Trichomonas vaginalis is a causative agent of trichomonalis, the most common protozoan infection in humans. It can infect the vagina and urethra in women, and sometimes the prostate gland in men. The duration of survival of T. vaginalis in transport medium is fairly limited. The organisms die rapidly when dried on a swab; an alternative approach is to place the loaded swab promptly into a tube of Trichomonas Culture Medium supplemented with horse serum, penicillin and streptomycin. Media for cultivation of T. vaginalis basically provide essential salts, nutrients, reducing agents and antibiotics to inhibit bacterial growth in the absence or in low concentration of oxygen. Johnson and Trussell (1) recommended CPLM (Cystine-Peptone-Liver infusion- Maltose) Medium. This medium was further modified without agar and methylene blue (2). Under strictly anaerobic conditions, this medium supports growth from a single protozoan. Under aerobic conditions, massive inocula are required. T. vaginalis is an anaerobe and contains no catalase. Modified CPLM HiVeg™ Medium Base is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ peptone and HiVeg™ extract No. 2 in the medium provide nitrogenous compounds and other essential nutrients. L-cystine hydrochloride acts as a reducing agent. Cystine is not essential when cultures are incubated anaerobically but it assists the maintenance of anaerobiosis. The antibiotics inhibit bacterial growth and supports growth from a single protozoon under strictly anaerobic conditions.
Type of specimen
please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further wet mount examination of infected material should be done.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Brownish yellow coloured clear solution without any precipitate
Reaction
Reaction of 5.6% w/v aqueous solution at 25°C. pH : 6.0±0.2
pH
5.80-6.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for upto 4 days.
| Organism | Growth |
|---|---|
| Trichomonas vaginalis ATCC 30001 | good-luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Modified CPLM HiVeg™ Medium Base (Trichomonas Modified CPLM HiVeg™ Medium Base) |
|---|---|
| SKU | MV460 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Johnson G. and Trussell R. E., 1943, Proc. Soc. Exp. Biol., 54:245. 2.Mackie and McCartneys Practical Medical Microbiology, 1989, 13th Ed. , Vol. 2, Churchill Livingstone, London. |
| Customized Product Available | No |
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