HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended with or without added blood for culturing fastidious microorganisms.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate No. 1 | 10.000 |
| HiVeg™ extract | 3.000 |
| Yeast extract | 1.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 34.0 grams in 950 ml purified/distilled water.Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For preparing Blood Agar cool the autoclaved medium to 45-50°C and aseptically add 5% v/v sterile defibrinated blood. Mix thoroughly, avoiding air bubbles and pour into sterile Petri plates.
Principle And Interpretation
Tryptose Blood Agar Base w/Yeast Extract, HiVeg™ is a tryptose based medium that can be used for the cultivation of fastidious organisms (1,2), on supplementation with blood. This medium is devoid of dextrose and therefore useful in determining the haemolytic reactions.
Tryptose Blood Agar Base w/ Yeast Extract, HiVeg™ provides additional nutrients (yeast extract) to the fastidious organisms. Tryptose Blood Agar Base w/ Yeast Extract, HiVeg™ can be used as a general-purpose medium without supplementation of blood. This medium can be used to determine the heamolytic reactions of fastidious organisms. The four different types of haemolysis observed are as follows:
a) α-haemolysis: partial lysis of the erythrocytes surrounding a colony, causing a gray green or brownish discolouration in the media.
b) β-haemolysis: complete lysis of the red blood cells surrounding a colony, causing a clearing of blood from the medium.
c) γ- haemolysis: no haemolysis and consequently, no colour change of the medium surrounding a colony. Organisms showing no haemolysis are generally termed non-hemolytic rather than gamma haemolytic.
d) α-prime or wide zone alpha: a small zone of intact erythrocytes immediately adjacent to the colony, with a zone of complete red cell haemolysis surrounding the zone of intact erythrocytes. This type of haemolysis may be confused with beta haemolysis (3).
Tryptose Blood Agar Base w/ Yeast Extract, HiVeg™ is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ hydrolysate No. 1, HiVeg™ extract and yeast extract provide nitrogenous and carbonaceous compounds, sulphur, vitamin B complex and trace elements essential for bacterial metabolism. Blood provides additional nutrients and serves as a base to study haemolytic reactions. This medium not only keeps the blood cells in a good state but also help in forming distinct haemolysis. Tryptose Blood Agar Base w/ Yeast Extract, HiVeg™ favours the good growth of Neisseria meningitides and Streptococcus pneumoniae. However, it can be used with or without blood supplementation.
Type of specimen
Isolated Microorganism
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Limitations :
- Perform biochemical test for further identification (4).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Basal medium: Yellow coloured clear to slightly opalescent gel forms in Petri plates. After addition of 5% v/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates.
Reaction
Reaction of 3.4% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed with added 5% v/v sterile defibrinated blood, after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth w/o blood | Recovery w/o blood | Growth w/ blood | Recovery w/ blood | Haemolysis |
|---|---|---|---|---|---|---|
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant | >=70% | luxuriant | >=70% | none |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% | beta |
| Staphylococcus epidermidis ATCC 12228 (00036*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% | gamma |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant | >=70% | luxuriant | >=70% | alpha |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=70% | luxuriant | >=70% | beta |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Tryptose Blood Agar Base w/ Yeast Extract, HiVeg™ |
|---|---|
| SKU | MV450 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Casman E. P., 1942, J. Bacteriol., 43:33. 2.Casman E. P., 1947, Am. J. Clin. Pathol., 17: 281. 3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore. 4.Koneman E. W., Allen S. D., Janda W. M., Schreckenberger P. C. Winn W. C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4 th Ed., J. B. Lippinccott Company |
| Customized Product Available | No |
Write Your Own Review
Blood Agar Base (Infusion Agar)
As low as
€0.00
Columbia Blood Agar Base
As low as
€0.00
Tryptose Blood Agar Base w/ Yeast Extract
As low as
€0.00
Anaerobic Tryptone Soya Agar
As low as
€0.00
Upsell Products
BHI Agar Slant
As low as
€0.00
BHI Agar
As low as
€0.00
Columbia 5% Sheep Blood Agar Plate
As low as
€0.00
BHI Agar Plate
As low as
€0.00
BHI Agar Plate w/ Blood
As low as
€0.00
Anaerobic Blood Agar Plate
As low as
€0.00