HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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HI Agar, HiVeg® (Heart Infusion Agar, HiVeg®)
Recommended for the isolation and cultivation of a wide variety of fastidious organisms. It is also used as a base for preparation of blood agar in determining haemolytic reactions.
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Intended Use
Recommended for the isolation and cultivation of a wide variety of fastidious organisms. It is also used as a base for preparation of blood agar in determining haemolytic reactions.
Composition**
| Ingredients | g / L |
|---|---|
| HiVeg® infusion | 10.000 |
| HiVeg® hydrolysate No. 1 | 10.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40.0 grams in 1000 ml of purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired 5% v/v sterile defibrinated blood may be added. Mix well and pour into sterile Petri plates.
Principle And Interpretation
HI Agar, HiVeg® is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. Fastidious organisms having exacting nutritional requirement could be cultivated on infusion media, as demonstrated by Huntoon (1). A liquid medium containing an infusion of meat was one of the first media used for the cultivation of bacteria. These infusion media need not be further supplemented by the addition of supplements for cultivation of fastidious bacteria (2). HI Agar, HiVeg®, containing HiVeg® infusion from (equivalent to infusion from beef heart) is used for the isolation and cultivation of a wide variety of fastidious organisms (3). HI Agar, HiVeg® can also be used for the cultivation of Vibrio species (2,4). It can also be supplemented with glucose, horse serum and antibiotics for the cultivation a wide variety of organisms (3). It is used for mass cultivation of organisms for preparation of vaccines. On supplementation of blood, HI Agar, HiVeg® can be used to study haemolytic reactions (6). This medium was used for isolation and enumeration of haemolytic Streptococci in milk (5). HiVeg® infusion and HiVeg® hydrolysate No. 1 infusion provide nutritional requirements for the pathogenic bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Food and dairy samples - Milk, meat samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (4,7). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Basal medium: Light yellow coloured, clear to slightly opalescent gel After addition of 5-7% w/v sterile defibrinated blood : Cherry red coloured, opaque gel forms in Petri plates
Reaction
Reaction of 4.0% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed with added 5-7% w/v sterile defibrinated blood, after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth w/o blood | Recovery w/o blood | Growth with blood | Recovery with blood | Haemolysis |
|---|---|---|---|---|---|---|
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant | >=70% | luxuriant | >=70% | beta |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant | >=70% | luxuriant | >=70% | none |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good | 50-70% | luxuriant | >=70% | alpha |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good | 50-70% | luxuriant | >=70% | beta |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% | beta |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | HI Agar, HiVeg® (Heart Infusion Agar, HiVeg®) |
|---|---|
| SKU | MV169 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,Washington D.C. 2.Atlas R. M., 2004, Handbook of Microbiological Media, 3rd Ed., CRC Press. 3.Diagnostic Procedures and Reagents, 1950, 3rd Edition, 13. 4.FDA Bacteriological Analytical Manual, 8th Ed., AOAC International, Gaithersburg, MD. 5.Huntoon F. M., 1918, J. Inf. Dis., 23:169. 6.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 7.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1 |
| Customized Product Available | No |
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