HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Semisolid IMRV HiVeg™ Medium Base
Semisolid IMRV HiVeg Medium Base is used for simultaneous enrichment as well as isolation of motile Salmonella from other competitive organisms.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 13.5 |
| HiVeg peptone | 13.5 |
| Saccharose | 7.5 |
| Lactose | 0.5 |
| Ammonium ferric sulphate | 0.2 |
| Sodium thiosulphate | 0.8 |
| Potassium dihydrogen phosphate | 1.47 |
| Magnesium chloride | 10.91 |
| Malachite green | 0.037 |
| Bromo cresol purple | 0.08 |
| Agar | 2.7 |
Final pH (at 25°C) 5.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Technical Data
Product Code: MV1427
Vegetable based (Code MV): HiVeg hydrolysate, HiVeg peptone
Animal based (Code M): M1427, Casein enzymic hydrolysate, Peptic digest of animal tissue
Recommended for: Simultaneous enrichment as well as isolation of motile Salmonella from other competitive organisms.
Reconstitution: 51.2 g/l
Quantity on preparation (500g): 9.76 L
pH (25°C): 5.5 ± 0.2
Supplement: IMRV/RV Selective Supplement (FD193).
Sterilization: Boiling (DO NOT AUTOCLAVE)
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 51.2 grams in 1000 ml distilled water. Heat with stirring to dissolve the medium completely. DO NOT AUTOCLAVE / DO NOT OVERHEAT. Cool to 45°C and aseptically add rehydrated contents of 1 vial of IMRV/ RV Selective Supplement (FD193). Mix well and pour into sterile petri plates.
Note: The motility of Salmonellas can be drastically reduced when the agar surface becomes too dry. Hence the plates should be well dried before use. If visible moisture occurs on the lid of the plates or the surface of agar, it must be removed. While incubation, incubate the plates aerobically in an upright position for no longer than 24 hours at 42°C.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate and HiVeg peptone in place of Casein enzymic hydrolysate and Peptic digest of animal tissue respectively, making the medium free of BSE/TSE risks. Semisolid IMRV HiVeg Medium Base is used for simultaneous enrichment and isolation of motile Salmonella from food and environmental samples. This medium, like the conventional medium is a diagnostic medium distinguishing motile Salmonella from non motile forms (1, 2). Also Salmonella can be identified from a mixed culture of different gram negative organisms. Addition of Novobiocin as a supplement and malachite green in the medium selectively inhibits most gram-positive organisms. Salmonella generally survives a little high osmotic pressure (due to MgCl₂ (magnesium chloride) in the medium), grows at slightly low pH and are resistant to malachite green compared to other bacteria. Saccharose, Lactose and Bromocresol purple differentiates Salmonella from lactose and saccharose (sucrose) fermenting organisms. Ammonium ferric sulphate and Sodium thiosulphate are indicators of H₂S (hydrogen sulphide) production. Thiosulphate is reduced to this H₂S by several species of bacteria and H₂S then combines with ferric ions to produce the insoluble black precipitate of ferrous sulphide as indicated by formation of greyish black colour at the centre of the colony.
Quality Control
Appearance of powder: Light yellow coloured, may have slightly opalescent tinge, homogeneous, free flowing powder.
Gelling: Semisolid, comparable with 0.27% Agar gel.
Colour and Clarity: Dark green coloured, clear to slightly opalescent semisolid medium forms in petriplates.
Reaction: Reaction of 5.12% w/v aqueous solution is pH 5.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 42°C for 18-24 hours, when one drop of culture is inoculated in the center of the medium plate.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Motility |
|---|---|---|---|
| Citrobacter freundii (8090) | 10²-10³ | inhibition | - |
| Pseudomonas aeruginosa (9027) | 10²-10³ | inhibition | - |
| Salmonella serotype Typhimurium (14028) | 10²-10³ | good | + * |
Key: * = development of purple halos of growth originating from the inoculation spot.
References
- Vander Zee H, and Van Netten P. 1992 Proc. Symp. "Salmonella and Salmonellosis". Ploufragan; 69.
- Puzickova, V; Karpiskova, R. and Pakrova, E. 1996. Vet Med. Praha. 41 (9): 283-288.
Prepared from GMO free Vegetable proteins replacing Animal based peptones. Freedom from BSE/TSE worries.
| Product Name | Semisolid IMRV HiVeg™ Medium Base |
|---|---|
| SKU | MV1427 |
| Customized Product Available | No |
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