HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Soyabean HiVeg Broth Base with supplement is recommended for enrichment and isolation of Escherichia coli 015:H7 from food samples.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 17.0 |
| Papaic digest of soyabean meal | 3.0 |
| Synthetic detergent No. I | 1.12 |
| Dextrose | 2.5 |
| Sodium chloride | 5.0 |
| Dipotassium hydrogen phosphate | 4.0 |
Final pH (at 25°C) 7.3 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1286 | M1286 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
| Synthetic detergent No. I | Bile salts mixture |
Recommended for
: Enrichment and isolation of Escherichia coli 015:H7 from food samples.
Reconstitution
| Quantity on preparation (500g) | : 32.62 g/l |
| 15.32 L | |
| pH (25°C) | : 7.3 ± 0.2 |
| Supplement | : Novobiocin Supplement (FD096) |
| Sterilization | : 121°C / 15 minutes. |
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 16.31 grams in 500 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 2 vials of Novobiocin Supplement (FD096). Mix well and dispense as desired.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which makes the medium free of BSE/TSE risks. Soyabean HiVeg Broth Base is the modification of Soyabean Bile Broth which is formulated as recommended by FDA (1) for the enrichment and isolation of Escherichia coli 015:H7.
HiVeg hydrolysate, Papaic digest of soyabean meal provide carbonaceous, nitrogenous compounds and other essential growth nutrients. Dextrose is the fermentable carbohydrate and energy source. Synthetic detergents No. I inhibits gram-positive bacteria. Sodium chloride maintains osmotic equilibrium while phosphate buffers the medium well. Novobiocin renders the medium selective. Whenever low levels of Escherichia coli 0157:H7 are suspected, the food is enriched in Soyabean HiVeg Broth Base and further plated on selective medium as MacConkey Sorbitol HiVeg Agar (MV298) for isolation and identification.
Blend 25 grams food sample to be tested in 224 ml Soyabean HiVeg Broth Base and incubate on shaker (about 100 rpm) for 18-24 hours at 37°C. Prepare dilution of the enrichment culture with phosphate buffer and spread 0.1 ml of each dilution on MacConkey Sorbitol HiVeg Agar (MV298) Plates and incubate at 43°C for 24 hours.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Light amber coloured clear solution without any precipitate.
Reaction
Reaction of 3.3% w/v aqueous solution is pH 7.3 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Recovery* |
|---|---|---|
| Escherichia coli (25922) | 10²-10³ | good |
| Escherichia coli 015:H7 | 10²-10³ | luxuriant |
| Staphylococcus aureus (25923) | 10²-10³ | none |
| Enterococcus faecalis (29212) | 10²-10³ | none |
Key: * = On MacConkey Sorbitol HiVeg Agar (MV298)
References
- Bacteriological Analytical Manual, 1995, 8th ed., Food and Drug Administration, AOAC International, Gaithersburg, USA.
| Product Name | Soyabean HiVeg™ Broth Base |
|---|---|
| SKU | MV1286 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, D.C. |
| Customized Product Available | No |
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