HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended for isolation and identification of Salmonellae.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 11.6 |
| Synthetic detergent No. II | 5.0 |
| Sodium chloride | 6.4 |
| Calcium carbonate | 20.0 |
| Potassium tetrathionate | 20.0 |
| Brilliant green | 0.07 |
| Final pH (at 25°C) | 7.0 ± 0.2 (after sterilization) |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 63.0 grams in 1000 ml distilled water. Heat just to boiling. DO NOT REHEAT. Dispense as desired.
Due to presence of calcium carbonate, the prepared medium forms opalescent solution with white precipitate.
Reconstitution: 63.0 g/l
Quantity on preparation (500g): 7.93 L
Supplement: None
Sterilization: Boiling (DO NOT AUTOCLAVE)
Principle and Interpretation
Tetrathionate Brilliant Green HiVeg Broth is prepared by completely replacing animal based peptones by vegetables peptones. Tetrathionate Brilliant Green HiVeg Broth is the modification of Tetrathionate Brilliant Green Bile Broth which is prepared for isolation and identification of Salmonella species from foods, water and other materials of sanitary importance.
HiVeg peptone provide nitrogenous nutrients to the Salmonellae. Brilliant green and synthetic detergent No. II inhibit both gram-positive as well as some selected gram-negative organisms. Potassium tetrathionate inhibits normal flora of faecal specimens. Sodium chloride helps in maintaining osmotic equilibrium. Calcium carbonate absorbs and neutralises the toxic metabolitics.
After incubation streak onto differential medium like MacConkey Agar (MV082) for isolation and identification. Tetrathionate Brilliant Green HiVeg Broth is not suitable for growth of Salmonella serotype Typhi and Salmonella serotype Paratyphi (1).
Quality Control
Appearance of powder: Greenish yellow coloured, homogeneous, free flowing powder.
Colour and Clarity
Bluish green coloured, opalescent solution with white precipitate. On standing the precipitate settles down.
Reaction
Reaction of 6.3% w/v aqueous solution after sterilization is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth* | Recovery | Colour of colony* |
|---|---|---|---|---|
| Escherichia coli (25922) | 102-103 | fair-good | >20% | pink-red |
| Salmonella serotype Enteritidis (13076) | 102-103 | luxuriant | >50% | colourless |
| Salmonella serotype Typhimurium (14028) | 102-103 | luxuriant | >50% | colourless |
| Staphylococcus aureus (25923) | 102-103 | inhibited | 0% | - |
Key: * on MacConkey HiVeg Agar (MV082)
Storage and Shelf Life
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
References
- MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
| Product Name | Tetrathionate Brilliant Green HiVeg™ Broth |
|---|---|
| SKU | MV1255 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg), Lactose |
| Packaging type | HDPE |
| References | 1. Mueller L., 1923, C. R. Soc. Biol., (Paris), 89, 434. |
| Customized Product Available | No |
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