HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
YT HiVeg Broth is used for the cultivation of recombinant strains of Escherichia coli.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 16.0 |
| Yeast extract | 10.0 |
| Sodium chloride | 5.0 |
| Final pH (at 25°C) | 7.0 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 31.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressue (121°C) for 15 minutes.
Principle and Interpretation
YT HiVeg Broth is prepared by using HiVeg hydrolysate which is free from BSE/TSE risks. YT HiVeg Broth is the modification of YT Broth which is recommended for use in the cultivation of recombinant strains of Escherichia coli (1-3) and also for propagating M13 bacteriophages (2). YT HiVeg Broth is a nutritionally rich growth medium containing HiVeg hydrolysate and yeast extract which supply nitrogenous compounds, vitamin B complex and other essential nutrients and co-factors. Sodium chloride helps in maintaining the osmotic equilibrium of the medium. Components of this medium provide essential nitrogenous compounds, for enhanced reproduction of bacteriophage without exhausting the host. Escherichia coli grows more rapidly as this medium provides nutrients that cells would otherwise have to synthesize.
Product Information
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1251 | M1251 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Specifications
| Recommended for | Cultivation of recombinant strains of Escherichia coli |
| Reconstitution | 31.0 g/l |
| Quantity on preparation (500g) | 16.12 L |
| pH (25°C) | 7.0 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and clarity: Light amber coloured, clear solution without any precipitate.
Reaction: Reaction of 3.1% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response: Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli (23724) | good-luxuriant |
| Escherichia coli (53868) | good-luxuriant |
| Product Name | YT HiVeg™ Broth |
|---|---|
| SKU | MV1251 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Miller H., 1987, Meth. Enzymol; 152, 145. |
| Customized Product Available | No |
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