HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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LPM (Lithium Phenylethanol Moxalactam) HiVeg® Agar Base is recommended for isolation and cultivation of Listeria monocytogenes from food and dairy products.
Composition ** :
| Ingredients | Grams/Litre |
|---|---|
| HiVeg® hydrolysate | 5.0 |
| HiVeg® peptone | 5.0 |
| HiVeg® extract | 3.0 |
| Glycine anhydride | 10.0 |
| Lithium chloride | 5.0 |
| Sodium chloride | 5.0 |
| Phenyl ethyl alcohol | 2.5 |
| Agar | 15.0 |
Final pH (at 25°C) 7.3± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile :
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1228 | M1228 |
| HiVeg® hydrolysate | Casein enzymic hydrolysate |
| HiVeg® peptone | Peptic digest of animal tissue |
| HiVeg® extract | Beef extract |
Recommended for : Isolation and cultivation of Listeria monocytogenes from food and dairy products,
Reconstitution
Reconstitution: 50.5 g/l
Quantity on preparation (100g): 1.98 L
pH (25°C): 7.3± 0.2
Supplement: Moxalactam Supplement (FD151)
Sterilization: 121°C / 12 minutes.
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions :
Suspend 50.5 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 12 minutes. Cool to 50°C and aseptically add rehydrated contents of 1 vial of Moxalactam Supplement (FD151). Mix well before pouring into sterile petri plates.
Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin, wash with plenty of water immediately.
Principle and Interpretation :
LPM HiVeg® Agar Base is prepared by using HiVeg® peptone, HiVeg® hydrolysate and HiVeg® extract which is free of BSE/TSE risks associated with animal based peptones. LPM HiVeg® Agar Base is the modification of LPM Agar Base which is modified McBride Agar developed by Lee and McClain (1) for the isolation of Listeria monocytogenes.
HiVeg® hydrolysate, HiVeg® peptone and HiVeg® extract provides all essential nutrients for metabolism. Glycine anhydride, lithium chloride and phenyl ethyl alcohol suppress gram-positive cocci and gram-negative rods. Moxalactam inhibits both gram-positive and gram-negative bacteria including Staphylococci, Proteus and Pseudomonas species. Listeria monocytogenes show blue-green irridescence when examined with oblique transmitted light (2, 3).
Quality Control :
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Light yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 5.05% w/v aqueous solution is pH 7.3± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 24 - 48 hours.
Organisms (ATCC)
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 102-103 | inhibited | 0% |
| Listeria monocytogenes (19111) | 102-103 | good-luxuriant | >50% |
| Listeria monocytogenes (19112) | 102-103 | good-luxuriant | >50% |
| Listeria monocytogenes (19117) | 102-103 | good-luxuriant | >50% |
| Pseudomonas aeruginosa (27853) | 102-103 | inhibited | 0% |
| Staphylococcus aureus (25923) | 102-103 | inhibited | 0% |
References :
- Lee and McClain, 1986, Appl. Environ. Microbiol., 52:1215.
- Bearns and Girard, 1959, Am. J. Med. Technol., 25:120.
- Borterelssi, Schtech and Albritton, 1985, Manual of Clinical Microbiology, Lennette, Balows, Hausler and Shadomy (Eds.), 4th ed., ASM, Washington, D.C.
| Product Name | LPM HiVeg® Agar Base |
|---|---|
| SKU | MV1228 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Lee W. H. and McClain D., 1986, Appl. Environ. Microbiol., 52:1215. |
| Customized Product Available | No |
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