HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Deoxycholate Lactose HiVeg Agar is a differential and slightly selective medium used for the isolation and enumeration of coliforms in water, waste water, milk and dairy products.
Recommended for: Isolation and enumeration of coliforms in water, waste water, milk and dairy products.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg special peptone | 10.0 |
| Lactose | 10.0 |
| Sodium chloride | 5.0 |
| Sodium citrate | 2.0 |
| Synthetic detergent No. III | 0.5 |
| Neutral red | 0.03 |
| Agar | 15.0 |
Final pH (at 25°C) 7.1 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 42.5 grams in 1000 ml distilled water. Mix well and heat to boiling to dissolve the medium completely. The medium requires no autoclaving if it is to be used at once. If the medium is to be stored, it should be sterilized at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING.
Principle and Interpretation
This medium is prepared by using vegetable peptones in place of animal based peptones that makes the medium free of BSE/TSE risks. This is the modification of Leifson (1) and prepared according to the formula in Standard Methods for Examination of Dairy Products (2) and Water and Wastewater (3) and food (4) for the detection of coliform bacilli.
This medium is selective against gram positive organisms which are inhibited by optimum concentration of synthetic detergent No. III and sodium citrate in the medium. It helps in differentiating between lactose fermentating and non lactose fermentating enteric bacilli. The lactose fermenters utilize lactose and produce acid which is indicated by pH indicator, neutral red. Lactose fermenters show pink coloured colony, while the lactose non fermenters form colourless colonies.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV066 | M066 |
| HiVeg special peptone | Peptone Special |
| Synthetic detergent No. III | Sodium deoxycholate |
Reconstitution
42.5 g/l
Quantity on preparation
- (500g): 11.76 L
- (100g): 2.35 L
pH (25°C)
7.1 ± 0.2
Supplement
None
Sterilization
Boiling or 121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Colour and Clarity
Reddish orange coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 4.25% w/v aqueous solution is pH 7.1 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C. for 18 - 24 hours
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour of Colony |
|---|---|---|---|
| Bacillus subtilis (6633) | 10²-10³ | inhibited | - |
| Ent. aerogenes (13048) | 10²-10³ | good - luxuriant | pink |
| Enterococcus faecalis (29212) | 10²-10³ | inhibited | - |
| Escherichia coli (25922) | 10²-10³ | good - luxuriant | pink |
| S. serotype Typhimurium (14028) | 10²-10² | good - luxuriant | colourless |
Quality Control
Appearance of powder
Light pink coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
References
- Leifson, 1935, J. Path. Bact., 40:581.
- Richardson (Ed.), 1985, Standard Methods for the Examination of Dairy Products, 15th ed., APHA, Wasington, D.C.
- Greenberg A. E., Trussell R. R. and CClesceri L. S. (Eds.) 1985, Standard Methods for the Examination of Water and Wastewater, 16th ed., APHA, Wasington, D.C.
- Speck M. (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd ed., APHA, Washington, D.C.
| Product Name | Deoxycholate Lactose HiVeg™ Agar |
|---|---|
| SKU | MV066 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg), Lactose |
| Packaging type | HDPE |
| References | 1.Leifson, 1935, J. Path. Bact., 40:581. 2.Richardson (Ed.), 1985, Standard Methods for the Examination of Dairy Products, 15th ed., APHA, Washington, D.C. 3.Greenberg A. E., Eaton A. D., Clesceri L. S., (Eds.), 1998, Standard Methods for the Examination of Water and Waste Water,20th Ed., APHA, Washington, D.C. 4.Speck M. (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd ed., APHA, Washington,D.C. |
| Customized Product Available | No |
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Deoxycholate Lactose Agar, Granulated
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