HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Actidione HiVeg Agar with Actidione / Base without Actidione is used for the enumeration and detection of bacteria in specimens containing large number of yeasts and moulds.
Product Type
Vegetable based (Code MV): MV400/MV058 HiVeg hydrolysate
Animal based (Code M): M400/M058 Casein enzymic hydrolysate
Composition
| Ingredients | MV400 Grams/Litre | MV058 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 5.00 | 5.00 |
| Yeast extract | 4.00 | 4.00 |
| Dextrose | 50.00 | 50.00 |
| Monopotassium phosphate | 0.55 | 0.55 |
| Potassium chloride | 0.425 | 0.425 |
| Calcium chloride | 0.125 | 0.125 |
| Magnesium sulphate | 0.125 | 0.125 |
| Ferric chloride | 0.0025 | 0.0025 |
| Manganese sulphate | 0.0025 | 0.0025 |
| Brom cresol green | 0.022 | 0.022 |
| *Actidione (Cycloheximide) | 0.01 | - |
| Agar | 15.00 | 15.00 |
Final pH (at 25°C) 5.5 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 75.26 grams of MV400 or 75.25 grams of MV058 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C. For (MV058) aseptically add 10 mg cycloheximide to the medium. Mix well before pouring into sterile petriplates.
Warning: *Actidione (Cycloheximide) is very toxic. Avoid skin contact or aerosal formation and inhalation.
Principle and Interpretation
This medium is prepared by replacing Casein enzymic hydrolysate with HiVeg hydrolysate. Actidione HiVeg Agar is the modification of Actidione Agar formulated by Green and Gray (1) which can be used for microbiological investigation during brewing and baking. Actidione (Cycloheximide) at a concentration of 0.001% permits the growth of bacteria and inhibits the growth of most yeasts and moulds except dermatophytes. This medium may be used for the estimation of bacterial contamination of pitching yeast. Addition of penicillin or streptomycin may be used for selective isolation of dermatophytes.
HiVeg hydrolysate acts as source of nitrogen while yeast extract serves as a rich reservoir of vitamins. Dextrose in high amount along with mineral salts at acidic pH favour sugar fermentation.
Supplement
Cycloheximide for (MV058)
Quantity on Preparation
For 500g: 6.64 L
Storage
(MV400): Dry Medium and Prepared Medium 2 - 8°C.
(MV058): Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of Powder: Light green coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Greenish blue coloured, clear to slightly opalescent gel forms in petri plates.
Reaction: Reaction of 7.53% w/v aqueous solution is pH 5.5 ± 0.2 at 25°C.
Cultural Response: Cultural characteristics observed after an incubation at 30°C for 40-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 10² | good-luxuriant | >50% |
| Lactobacillus fermentum (9338) | 10² | good-luxuriant | >50% |
| Proteus mirabilis (25933) | 10² | good-luxuriant | >50% |
| Saccharomyces cerevisiae (9763) | 10² | inhibited | 0% |
| Saccharomyces uvarum (9080) | 10² | inhibited | 0% |
| Product Name | Actidione HiVeg™ Agar Base w/o Actidione® |
|---|---|
| SKU | MV058 |
| Customized Product Available | No |
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