HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Antibiotic HiVeg Assay Medium No. 2 (Base HiVeg Agar) is used for microbiological assay of antibiotics.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg peptone | 6.000 |
| HiVeg extract | 1.500 |
| Yeast extract | 3.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Advice: Recommended for the microbiological assay of Bacitracin, Cephalexin, Cephaloglycin, Cephaloridine, Cephalothin, Cloxacillin, Cycloserine, Dicloxacillin, Methicillin, Nafcillin, Novobiocin, Oxacillin, Penicillin-G, Penicillin, Rifampicin, Spiramycin
Principle And Interpretation
Antibiotic HiVeg Assay Medium No. 2 is prepared by incorporating vegetable peptones in place of animal peptones, making the medium BSE, TSE risks free. It can be used for the same purpose of Antibiotic Assay Medium No. 2 and is recommended for use as base agar for microbiological agar diffusion assays for wide variety of antibiotics. Agar diffusion assays can be performed by cylinders, punched-hole or paper disc tests. The equivalent animal based medium is identical numerically with the name assigned by Grove and Randall (1) that is equivalent to the Antibiotic Assay Medium No. B of Indian Pharmacopoeia (2). HiVeg Peptone, yeast extract and HiVeg extract provide the nitrogenous, vitamins and mineral requirement for the growth of test organisms. This medium provides solidified substratum for growth of organism and supports the overlayering of soft agar.
This medium is widely used to prepare the base layer in the microbiological assay of antibiotics such as Bacitracin, Cephalexin, Cephalothin, Cephapirin, Cloxacillin, Dicloxacillin, Methicillin, Nafcillin, Oxacillin, Chloramphenicol, Novobiocin and Penicillin. To perform the antibiotic assay the Antibiotic HiVeg Assay medium No.2 is used as base agar. This medium should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, Antibiotic assay medium No.1 as seed agar, inoculated with the standardized culture can be overlaid. Even distribution of the layer is important.
Note: For Antibiotic Assay Methods and Selection of Antibiotic HiVeg Assay Medias, Refer Section Antibiotic HiVeg Assay Media.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 2.55% w/v aqueous solution at 25°C. pH : 6.6±0.2
pH
6.40-6.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Basal layer |
|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | >=70% | Spiramycin |
| Micrococcus luteus ATCC 10240 | 50-100 | luxuriant | >=70% | Bacitracin |
| Staphylococcus aureus ATCC 9144 | 50-100 | luxuriant | >=70% | Tylosin |
| Staphylococcus aureus ATCC 29737 | 50-100 | luxuriant | >=70% | Amikacin, Cephalothin, Cephapirin, Chlortetracycline, Nafcillin, Oxytetracycline, Tetracycline, Cloxacillin Rolitetracycline, Cycloserine, Demeclocycline, Doxycycline, Kanamycin, Methacycline |
| Staphylococcus epidermidis ATCC 12228 | 50-100 | good-luxuriant | >=70% | Novobiocin |
| Klebsiella pneumoniae ATCC 10031 | 50-100 | luxuriant | >=70% | Capreomycin, Streptomycin, Troleandomycin |
| Enterococcus hirae ATCC 10541 | 50-100 | luxuriant | >=70% | Gramicidin, Thiostrepton, Tobramycin |
| Escherichia coli ATCC 10536 | 50-100 | luxuriant | >=70% | Chloramphenicol, Spectinomycin |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic HiVeg™ Assay Medium No. 2 (Base HiVeg™ Agar) |
|---|---|
| SKU | MV005 |
| Application/ Industry | Pharmaceutical |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Regulatory | IP |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Grove and Randall, 1955, Assay Methods of Antibiotics Medical Encyclopedia, Inc. New York.1. 2. Indian Pharmacopoeia 2017, Minstry of Health and Family Welfare, Govt. of India, Delhi. 3. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 4. Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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