HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use:
Recommended for the isolation and cultivation of Neisseria species from clinical specimens.
Composition**
| Ingredients | g/L |
|---|---|
| Tryptone | 7.500 |
| HM Peptone # | 7.500 |
| Dipotassium hydrogen phosphate | 4.000 |
| Potassium phosphate | 1.000 |
| Corn starch | 1.000 |
| Sodium chloride | 5.000 |
| Agar | 12.000 |
FO Growth Supplement (FD022) 10.000
Vitamino Growth Supplement (FD025) 1.0 vial
| Ingredients | Concentration |
|---|---|
| Part I | |
| Vitamin B12 | 0.100mg |
| L-Glutamine | 100mg |
| Adenine sulphate | 10mg |
| Guaninine hydrochloride | 0.300mg |
| p-Aminobenzoic acid (PABA) | 0.130mg |
| L-Cystine | 11mg |
| NAD (Coenzyme I) | 2.500mg |
| Cocarboxylase | 1mg |
| Ferric nitrate | 0.200mg |
| Thiamine hydrochloride | 0.030mg |
| Cysteine hydrochloride | 259mg |
| Part II (Rehydrating fluid) | |
| Dextrose | 1g |
| Distilled water | 10ml |
VCAT Supplement (FD353) 2vial
| *Ingredients | Concentration |
|---|---|
| Vancomycin | 1.0 mg |
| Colistin sulphate | 3.75 mg |
| Amphotericin B | 0.5 mg |
| Trimethoprim | 1.5 mg |
**Formula adjusted, standardized to suit performance parameters
#- Equivalent to Meat peptone
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Majority of gonococcal infections are uncomplicated lower genital tract infection caused by direct infection of the columnar epithelium of mucosal membranes. Neisseria gonorrhoeae is the causative agent of gonococcal infections. Most Neisseria strains have complex growth requirements; some strains may be exquisitely sensitive to fatty acids, necessitating the incorporation of soluble starch in the growth media (1).
Carpenter and Morton reported an improved medium to isolate Gonococci in 24 hours (2,3). Later on the efficiency of GC medium supplemented with hemoglobin and yeast concentrate was demonstrated for isolating gonococci (4). Subsequently Thayer and Martin Medium was developed for the primary isolation of Neisseria gonorrhoeae and Neisseria meningitidis from specimens containing mixed flora collected from throat, vagina, rectum and urethra (5,6). Thayer and Martin (6) used Vancomycin, Colistin and Nystatin. Martin and Lester (5) used an additional antibiotic Trimethoprim to make the medium selective.
Tryptone and HM Peptone supplies nitrogenous and carbonaceous compounds, long chain amino acids and other essential growth nutrients for the growth of fastidious organisms. Phosphates buffer the medium. Sodium chloride maintains the osmotic balance. For the cultivation of fastidious organisms the medium should be supplemented with essential growth factors supplied predominantly by yeast extract (FD027). This can be replaced with a chemically defined supplement containing essential growth factors available from yeast extract in Vitamino Growth Supplement (Twin Pack) (FD025). X-factor needed for the growth of fastidious Haemophilus species is provided by hemoglobin (FD022). Selective supplement inhibits accompanying bacteria.
Type of specimen
Clinical samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (7,8).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to nutritional variations, certain strains may show poor growth.
- Certain strains of Neisseria gonorrheae may be inhibited by antibiotics.
- An enriched non-selective medium must be used in parallel.
- Further biochemical and serological tests must be carried for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Martin Lewis Agar in 90mm disposable plate with smooth surface and absence of black particles/cracks/bubbles.
Colour of medium
Chocolate brown coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plate
Cultural response
Cultural characteristics observed in presence of 5-10% Carbon dioxide (CO2) and 70% humidity, after an incubation at 35-37°C for 40-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Haemophilus influenzae ATCC 19418 | 50-100 | good-luxuriant | >=50% |
| Neisseria gonorrhoeae ATCC19424 | 50-100 | good-luxuriant (with added antibiotic supplements) | >=50% |
| Neisseria meningitidis ATCC 13090 | 50-100 | good-luxuriant (with added antibiotic supplements) | >=50% |
| Streptococcus pyogenes ATCC19615 | 50-100 | good-luxuriant | >=50% |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant | >=50% |
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Martin Lewis Agar Plate |
|---|---|
| SKU | MP2085 |
| Customized Product Available | No |
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