HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended use
Recommended for enumeration of bacteria in milk and milk products, rinse waters, ice creams etc.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Peptone | 5.000 |
| Yeast extract | 3.000 |
| M-protein solids# | 1.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
#- Equivalent to Milk solids
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
The milk secreted in an uninfected cow's udder is sterile. Contamination of this milk can occur during milking, cooling and storage (1). Milk is an excellent medium for bacteria, yeast and moulds. Their rapid growth can cause marked deterioration, spoiling the milk for liquid consumption or manufacture into dairy products. Human infection can occur by consumption of such contaminated milk or milk products. Milk Agar is recommended for performing plate count tests on milk, rinse waters and dairy products. It is formulated as per the official medium described by Department of Health Memo (2). It is also recommended by EUROGLACE (EEC Ice Cream Industries) for the examination of ice cream (2).
Peptone and yeast extract provide essential nutrients while M-Protein solids are a source of casein. Dextrose is the carbon and energy source. Proteolytic bacteria will be surrounded by a clear zone, due to the conversion of casein into soluble nitrogenous compounds (3).
For milk, dilutions of 1/10, 1/100 and 1/1000 are prepared with 1/4 strength Ringer solution. 1 ml of each dilution is pipetted aseptically into sterile Petri plates to which 10 ml of sterile and cooled Milk Agar is added and mixed well. Plates should be poured within 15 minutes of dilution preparation. After solidification of medium the plates are allowed to stand for 1 hour before transferring to the incubator. Incubate at 35°C for 2 or 3 days at 30°C. Higher counts may be obtained after an incubation at 22°C and 30°C temperature rather than at 35°C (3,4,5). Count the colonies within 4 hours after the incubation and read it as per ml of sample.
Type of specimen
Milk and milk products, rinse waters, ice creams etc.
Specimen Collection and Handling:
For milk and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (6,7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions:
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's requirement.
- Further serological and biochemical testing is required for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Modified Plate Count Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/ bubbles
Colour of medium
Light yellow coloured medium
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacillus subtilis subsp. spizizenni ATCC 6633 (00003*) | 50-100 | good-luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | good-luxuriant | >=70% |
| Lactobacilus casei ATCC 9595 | 50-100 | good-luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant | >=70% |
| Serratia marcescens ATCC 8100 | 50-100 | good-luxuriant | >=70% |
*- Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | Modified Plate Count Agar Plate |
|---|---|
| SKU | MP163 |
| Customized Product Available | No |
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