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10X Tris-Tricine-SDS Gel Running Buffer
Tricine–SDS‐PAGE is commonly used to separate proteins of lower molecular weight. Laemmli SDS‐PAGE gel can separate high molecular weight proteins (20‐200 kDa) but proteins less than 20 kDa are not clearly separated and diffused even if higher acrylamide concentration (4‐20% gradient gels) are used. Tris‐Tricine‐SDS is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems.
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Buffers and Chemicals for Protein Electrophoresis#902790
Tricine–SDS-PAGE is commonly used to separate proteins of lower molecular weight. It is composed of 1M Tris, 1M Tricine and 1% SDS and is preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. It helps for low molecular weight proteins to be separated even in low % acrylamide gels.Lower acrylamide concentrations of Tricine gels help in easy transfer of hydrophobic proteins during Western blotting. This buffer is used as a cathode buffer.
| Product Name | 10X Tris-Tricine-SDS Gel Running Buffer |
|---|---|
| SKU | ML196 |
| Customized Product Available | No |
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