10X Tris acetate-SDS Running Buffer is used for Tris-Acetate Gels during protein electrophoresis. It resolves native proteins more effectively than the Tris-Glycine gel system. This buffer contains Tricine, Tris base and SDS mixed in a ratio to achieve pH 8.23. No acid or base is added to the buffer for this pH adjustment. This buffer is conveniently supplied as 10X concentrated stock solution and should be diluted to 1X with deionized water before usage
10X Tris acetate-SDS Running Buffer is used for Tris-Acetate Gels during protein electrophoresis.
It resolves native proteins more effectively than the Tris-Glycine gel system. This buffer contains 500mM Tris base, 500mM Tricine, 3.5mM SDS mixed in a ratio to achieve pH 8.23. No acid or base is added to the buffer for this pH adjustment. Mainly used for high molecular weight proteins.