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HiPurA® HP Fungal DNA Purification Kit (following bead beating method)

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MB576

This kit simplifies isolation of DNA from fresh fungal tissue with spin column procedure. The procedure is optimized for a maximum of 300 mg of wet‐weight of the starting material. The sample (fresh) is added to HiBead tubes along with HP Fungal Extraction Buffer. HP Fungal Extraction Buffer contains CTAB (Cetyltrimethylammonium bromide), a detergent used to break open cells and solubilize the contents. Chlorophyll and some denatured proteins are removed from green plant tissue in an organic chloroform‐ isoamylalcohol step, and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A. The flow‐through fraction is then mixed with a solution that enhances the binding of DNA to the column. The solution is then passed through HiElute Miniprep Spin Column (Capped) that is followed by washing steps to remove trace contaminants. High quality DNA is eluted in the Elution Buffer (ET) provided in the kit.

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Description

HiPurA® HP Fungal DNA Purification Kit provides a fast and easy method for purification of total DNA from fungus using the miniprep spin column. It is based on the advanced silica binding principle presented in a microspin format facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. It comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HP Fungal Extraction Buffer contains CTAB, a detergent used to break open cells and solubilize the contents. In this technique the sample is lysed and homogenized in a bead-beating step. Concentration of DNA can be estimated by agarose gel electrophoresis.

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