HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Neutralization Buffer A (for transfer to uncharged membranes)
Neutralization Buffer A is used for transfer of DNA bands from agarose gel to uncharged membrane (e.g. nitrocellulose) during Southern hybridization procedure. This buffer lowers the pH of DNA during the blotting process. If the DNA gel is depurinated prior to alkaline or nonalkaline transfer, exclusion of the neutralization step prior to transfer can reduce signal. Without a neutralization step, depurination continues in the gel.
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Reagents for Southern & Northern Blotting#902790
Neutrailzation Buffer A is used for transfer of DNA bands from agarose gel to uncharged membrane (e.g. nitrocellulose) during Southern hybridization procedure. This buffer lowers the pH of DNA during the blotting process. It is composed of 1M Tris and 1.5 M Sodium chloride and the pH is adjusted to 7.4.
| Product Name | Neutralization Buffer A (for transfer to uncharged membranes) |
|---|---|
| SKU | ML034 |
| Customized Product Available | No |
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