Denaturation Solution denatures the double-stranded DNA and as a result DNA can be efficiently transferred to nylon or nitrocellulose membrane and subsequently hybridized to a labeled probe during neutral Southern blotting procedure. When denaturation solution is used in conjunction with depurination solution, the phosphodiester backbone at the sites of depurination is hydrolysed which allows a very efficient transfer of large DNA to the membrane.
Denaturation Solution denatures the double-stranded DNA and as a result DNA can be efficiently transferred to nylon or nitrocellulose membrane and subsequently hybridized to a labeled probe during neutral Southern blotting procedure. When denaturation solution is used in conjunction with depurination solution, the phosphodiester backbone at the sites of depurination is hydrolysed which allows a very efficient transfer of large DNA to the membrane. It is composed of Sodium chloride and strong base Sodium hydroxide