HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Alkaline Transfer Buffer
Alkaline Transfer Buffer contains a strong base that denatures double-stranded DNA. This buffer allows the hydrolysis of the phosphodiester backbone at the sites of depurination during Southern blotting and as a result large DNAs are very efficiently transferred and covalently linked to the positively charged nylon membrane.
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Reagents for Southern & Northern Blotting#902790
Alkaline Transfer Buffer is mainly used to transfer DNA from agarose gels to charged nylon
membranes by capillary method during Southern hybridization method. Alkaline Transfer Buffer contains a strong base that denatures double-stranded DNA. This buffer allows the hydrolysis of the phosphodiester backbone at the sites of depurination during Southern blotting and as a result allowing large DNAs to be very efficiently transferred and covalently linked to the positively charged nylon membrane.
| Product Name | Alkaline Transfer Buffer |
|---|---|
| SKU | ML032 |
| Customized Product Available | No |
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