RNase T1, Recombinant

RNase T1 is a recombinant protein isolated through a series of purification steps and it is an endoribonuclease. This enzyme specifically cleaves single stranded RNA after the Guanosine residue site, producing a 3'phosphate terminal. It cleaves the phosphodiester bond between the 3'‐guanylic residue and the 5'‐OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'‐cyclic phosphate. The reaction products are 3'‐GMP and oligonucleotides with a terminal 3'‐GMP. The activity of RNase T1 does not require the involvement of metal ions and can be used for rapid analysis of the physical structure of target RNA.