The 5X Sucrose Gel Loading Dye should be mixed with DNA samples including genomic and plasmid DNA, PCR product, restriction fragments etc. to facilitate the loading of the samples into the wells of agarose gels. It contains two different dyes Xylene Cyanol FF and Bromophenol blue for two-color visual tracking of DNA migration during gel electrophoresis. This dye contains sucrose which ensures that the DNA in the sample becomes more viscous and forms a layer at the bottom of the well.
The 5X Sucrose Gel Loading Dye is a pre-mixed, ready-to-use loading buffer. It contains two different dyes Xylene Cyanol FF and Bromophenol blue for two-color visual
tracking of DNA migration during gel electrophoresis. This dye contains sucrose which ensures that the DNA in the sample becomes more viscous and forms a layer at the bottom of the well. It should be used by mixxing one volume of 5X Sucrose Gel Loading Dye to 4 volumes of DNA sample