5X Gel Loading Buffer

The 5X Gel Loading Buffer should be mixed with DNA samples including genomic and plasmid DNA, PCR product, restriction fragments etc. to facilitate the loading of the samples into the wells of agarose gels. It contains three different dyes Bromophenol blue, Xylene Cyanol FF and a proprietary magenta dye for three-color visual tracking of DNA migration during gel electrophoresis. This dye contains glycerol which ensures that the DNA in the sample becomes more viscous and forms a layer at the bottom of the well. Moreover, EDTA is included in this loading buffer which inhibits metal-dependent nucleases by binding divalent metal ions. Addition of one volume of 5X Gel Loading Buffer to 1 volume of DNA sample gives color and density to the sample which facilitates the loading. The three dyes of the 5X Gel Loading Buffer migrate in the same direction as the DNA samples providing an indicator of the electrophoretic progress. The magenta dye migrates much faster than Bromophenol Blue and Xylene cyanol.