MOPS is the common name for 3-(N-morpholino)propanesulfonic acid. It is a zwitterionic buffer which was developed by Good et al. in 1966. It is structurally analogous to MES and it's metal binding capacity is negligible. As the pKa value of MOPS is 7.20 it is an excellent buffer for many biological systems at near-neutral pH. This is a buffer of low ionic strength and prepared from highly pure nuclease free MOPS. Usage of this buffer during electrophoresis saves a lot of time as it is very convenient.
10X MOPS Electrophoresis Buffer contains MOPS, Sodium hydroxide, Sodium acetate and EDTA. designed for use in electrophoresis of agarose gels containing RNA
samples. This is a concentrated buffer and should be diluted to 1X before use.