HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Complete Supplement Mixture w/o HIS-URA
Complete supplement for amino acids to prepare appropiate media for selection of HIS3 and URA3 auxotrophic strains and transformants
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Saccharomyces cerevisiae and Schizosaccharomyces Pombe#902790
Intended Use
Complete Supplement Mixture w/o HIS-URA is used as a dropout supplement for all strains of Saccharomyces cerevisiae.
Composition
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 10.00 |
| L-Arginine HCl | 50.00 |
| L-Aspartic acid | 80.00 |
| L-Isoleucine | 50.00 |
| L-Leucine | 100.00 |
| L-Lysine HCl | 50.00 |
| L-Methionine | 20.00 |
| L-Phenylalanine | 50.00 |
| L-Threonine | 100.00 |
| L-Tryptophan | 50.00 |
| L-Tyrosine | 50.00 |
| L-Valine | 140.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 750 mg in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Complete Supplement Mixture w/o HIS-URA is used as a dropout supplement for all strains of Saccharomyces cerevisiae. Complete Supplement Mixture (CSM) is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. Furthermore, it contains uracil. CSM w/o HIS-URA does not contain histidine and uracil. This makes it a dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of histidine and uracil prototrophs and wild type strains of S. cerevisiae but histidine or uracil auxotroph cannot grow in this media. Therefore, CSM w/o HIS-URA can be used as drop-out formulation for the selection of auxotrophic requirements and transformants.
Quality Control
Appearance of Powder:
Light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity:
Light yellow coloured, clear solution without any precipitate.
Cultural Response:
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Complete Supplement Mixture w/o HIS-URA |
|---|---|
| SKU | G119 |
| Customized Product Available | No |
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