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Antibiotic Assay Medium J
A general purpose medium for cultivation of a wide variety of microorganisms and for sterility testing in pharmaceutical procedures in accordance with Indian Pharmacopoeia.
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Antibiotic assay medium No. 36 is used for inoculum preparation of Mycobacterium smegmatis for the aasay of Bleomycin in accordance with Indian Pharmacopoeia.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone # | 15.000 |
| Soya peptone # # | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| pH after sterilzation | 7.3±0.1 |
**Formula adjusted, standardized to suit performance parameters
# Pancreatic digest of casein
## Papaic digest of soyabean meal
Directions
Suspend 40 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.Cool to 45-50°C .Mix well and pour into sterile Petri plates.
Principle And Interpretation
Grove and Randall have elucidated the antibiotic assays and medias in their comprehensive treatise on antibiotic assays (1). Antibiotic assay Medium No. 36 is recommended for preparation of inoculum of Mycobacterium smegmatis for the assay of Bleomycin. This medium is also used for the cultivation of a wide variety of microorganisms and sterility testing of pharmaceutical preparations. (3). This medium is formulated in accordance with Indian Pharmacopoeia (2).
The combination of Tryptone and soya peptone makes this medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance of the medium
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light yellow coloured clear to slightly opalescent gel forms in Petri plates
pH: 7.20-7.40
Cultural Response
MM1666: Cultural characteristics observed after an incubation at 36-37.5°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Cultural Response | ||||
| Mycobacterium smegmatis ATCC 607 | 50-100 | luxuriant | >=50% | Bleomycin |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium J |
|---|---|
| SKU | MM1666 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Grove and Randall, 1955, Assay Methods of Antibiotics, Medical Encyclopedia, Inc. New York |
| Customized Product Available | No |
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