HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Fluid Lactose Medium (Lactose Broth) Medium 4
For detection of coliform bacteria in water, food, dairy products in accordance with Indian Pharmacopoeia.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Beef extract | 3.000 |
| Pancreatic digest of gelatin | 5.000 |
| Lactose monohydrate | 5.000 |
| pH after sterilization (at 25°C) | 6.9±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 12.75 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. For largerinocula (10 ml or more), concentrated medium may be prepared to account for medium dilution by the inoculum. Dispense in tubes containing inverted fermentation vial (Durhams tube) as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Fluid Lactose Medium is recommended by APHA in the performance and confirmation of the presumptive test for coliform bacteria in water (1), food (2), milk (3) and is recommended by IP (4). This medium can be used as an alternate to Lauryl Sulphate Broth in the presumptive test of the MPN of standard coliforms. This medium is also used for pre-enrichment of Salmonella for its detection in pharmaceutical raw materials.
Pancreatic digest of gelatin and beef extract supply essential nutrients to the organisms. Lactose monohydrate is a fermentable carbohydrate for the coliforms. Tubes of Lactose Broth are inoculated with dilutions of water or milk, etc. under test, and incubated at 35°C and examined for gas formation after 24 and 48 hours.
Members of the coliform group are defined as aerobic and facultative anaerobic gram-negative and non-sporing bacilli, which ferment lactose with gas formation within 48 hours at 35°C. In testing dairy products, Lactose Broth is used only in the completed test (3). Large water samples may require double strength Lactose Broth to minimize the final volume.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured clear solution
pH
6.70-7.10
Cultural Response
MM1003: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Gas |
|---|---|---|---|
| Enterobacter aerogenes ATCC 13048 | 50-100 | luxuriant | Positive reaction |
| Enterococcus faecalis ATCC 29212 | 50-100 | luxuriant | Negative reaction |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | Negative reaction |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | Positive reaction |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | Positive reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Fluid Lactose Medium (Lactose Broth) Medium 4 |
|---|---|
| SKU | MM1003 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Eaton A. D., Clesceri L. S. and Greenberg A W.,(Eds.), 2005, Standard Methods for the Examination of Water andWastewater, 21st ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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