HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Slant in Glass Tubes#4fa25a
Intended use
For isolation of dermatophytes and other pathogenic fungi.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Soya peptone | 10.000 |
| Dextrose (Glucose) | 10.000 |
| Selective mix | 0.550 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Streak the test inoculum aseptically into the slant and incubate at appropriate conditions.
Principle And Interpretation
Fungi that multiply on skin are called dermatophytes. Dermatomycosis is a general term for mycotic parasitism of the skin. These can be isolated on differential selective media containing generous amounts of glucose and antibiotics to suppress bacterial growth (1).
Fungobiotic Agar is used for isolation of pathogenic fungi from mixed microbial flora. Georg et al (2) recommended addition of two antibiotics cycloheximide and chloramphenicol for the primary isolation of dermatophytes and fungi which cause systemic disease as none of the dermatophytes are sensitive to these antibiotics but some fungi causing systemic disease may be inhibited by one or the other antibiotic (3). For this reason media without antibiotics must be used in parallel with Fungobiotic Agar.
Soya peptone and dextrose provide essential nutrients for fungal growth. Selective mix inhibits saprophytic fungi, certain yeasts and moulds (4, 5) and also has an inhibitory action on the accompanying bacteria (6, 7). Temperature of incubation affects the sensitivity of certain systemic pathogenic fungi to Selective mix (8). It is therefore recommended that incubation should be carried out at 25-30°C.
Type of specimen
Isolated microrganism
Specimen Collection and Handling
For samples follow appropriate techniques for handling specimens as per established guidelines
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before processing. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to varying nutritional requirements and resistance, certain isolated strains may show varying growth.
- Further biochemical tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Mycobio Agar in glass tube
Colour: Medium amber coloured opalescent slants.
Quantity of Medium: 8 ml of medium in glass tube.
Reaction: 6.30-6.70
Sterility test - Passes for release criteria
Cultural response
Cultural characteristics observed after an incubation at 25-30°C for 4-7 days.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Aspergillus brasiliensis ATCC 16404 ((00053)*) | >=104 | inhibited |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant |
| Candida tropicalis ATCC 1369 | >=104 | inhibited |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited |
| Staphylococcus epidermidis ATCC 12228 (00036*) | >=104 | inhibited |
| Trichophyton equinum ATCC 22443 | 50-100 | luxuriant |
| Trichophyton verrucosum ATCC 36058 | 50-100 | luxuriant |
Key: (#)- Formerly known as Aspergillus niger (*) - Corresponding WDCM numbers
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | Mycobio Agar Slant (Fungobiotic Agar Slant) |
|---|---|
| SKU | SL203 |
| Customized Product Available | No |
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