HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended for sample collection, transportation, detection of suspected Mucor species (after sporulation appear as black fungi) and Candida species (appears white in colour) causing Mucormycosis.
Kit contents
| 2 kits Sufficient for 2 samples |
10 kits Sufficient for 10 samples |
|
|---|---|---|
| 1. MP5476: Mucormycosis Selective Agar Plate | 2 plates | 10 plates |
| 2. MP5477: Candida Selective Agar Plate | 2 plates | 10 plates |
| 3. MS5478: HiFungal™ Transport medium w/swab (Sterile flocked nylon swab for specimen collection. | 2 nos. | 10 nos. |
Refer individual technical data sheets for more details.
Advantages
- A complete one stop solution for the collection and transport of sample and selective isolation of Mucor species (black fungus and Candida species (white fungus).
- MS5478: Collection and transport of sample of fungi.
- MP5476: Selective isolation of Mucor fungi inhibiting bacterial contamination and yeasts.
- MS5477: Selective isolation of Candida species inhibiting bacterial contamination and mycelial fungi.
- Lockable plates to ensure clinician safety.
Directions
- Cut open the pouch containing the swab for specimen collection
- Collect the specimen using standard techniques.
- After sample collection, insert the swab into the HiFungal™ Transport medium and break the swab at the breakpoint.
- Tighten the cap firmly.
- The specimen will be preserved during transportation and also the viability of the organisms will be maintained. After the transportation, the specimen should be inoculated in respective medium as soon as possible.
- MP5476- Mucormycosis Selective Agar Plate - selective cultivation of Mucor species (after sporulation appear as black fungi).
- MP5477- Candida Selective Agar Plate -selective isolation of Candida apecies (appears as white fungi)
- For cultivation of other fungi any general fungal medium as Sabouraud Dextrose Agar (GM063/MP063) or Potato Dextrose Agar (M096/ MP096) etc.
Principle And Interpretation
Mucormycosis (previously called zygomycosis) is a serious but rare fungal infection caused by a group of molds called mucormycetes (1). In most cases it is due to an invasion of the genera Rhizopus and Mucor, common bread molds (2). Effective recovery of microorganisms and its identification is dependent on a number of factors such as collection and transportation to the laboratory under conditions which allow maintenance of viability. This medium gives improved recovery of fungal cultures including yeasts and moulds. The recovery of mycelial fungi including Aspergillus, Mucor, Rhizopus is also well supported. The fungal cultures can be safely transported. Antibacterial components have been added to the transport medium to avoid bacterial contamination.
Mucormycosis selective Agar Plate is specifically designed to selectively promote the growth of Mucor species and inhibit the contaminating bacterial flora and yeast.
Candida Selective Agar Plate is specifically designed to selectively promote the growth of Candida species and inhibit the contaminating bacterial flora and mycelial fungi.
Type of specimen
Clinical samples: eye lesion, nasal swabs, other sites of infection
Specimen Collection and Handling
HiFungal™ Tranport medium w/swab (MS5478) which contains the transport medium along with the swab is used for specimen collection from the eye, nose, nasopharynx, other sites of infection and transported to the lab. can be used. (Refer directions).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro Diagnostic Use only. Read the label before opening the kit Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Microscopy, morphological tests, staining tests and further biochemical tests are recommended for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
MP5476 - Mucormycosis Selective Agar Plate
MP5477 - Candida Selective Agar Plate
MS5478 - HiFungal™ Transport Medium with sterile flocked nylon swab.
Sterility test
Passes release criteria
Cultural response
Viability of following organisms was established for a period of 48 hours. For selective cultivation of Mucormycosis fungi, the swab should be swabbed onto Mucormycosis Selective Agar Plate (MP5476) and for selective isolation of white fungi, Candida species, the swab must be swabbed onto Candida Selective Agar Plate (MP5477). For cultivation of fungi any general fungal medium such as Sabouraud Dextrose Agar, Potato Dextrose Agar etc can be used. The plates are then incubated at 25-30°C for 24-48 hours. Overincubation should be avoided.
Cultural Response
| Organism | Growth on Mucoromycosis Selective Agar Plate (MP5476) |
Growth on Candida Selective Agar Plate (MP5477) |
|---|---|---|
| Mucor racemosus isolate | Luxuriant (Black spores observed) | none-poor |
| Rhizopus oryzae MTCC 1987 | Luxuriant (Black spores observed) | none-poor |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | none-poor | none-poor |
| Candida albicans ATCC 10231 (00054*) | none-poor | Luxuriant |
| Trichophyton rubrum ATCC 28191 | none-poor | none-poor |
| Escherichia coli ATCC 25922 (00013*) | inhibited | inhibited |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | inhibited | inhibited |
| Pseudomonas aeruginosa ATCC 27853 (00087*) | inhibited | inhibited |
Key: (#) - Formerly known as Aspergillus niger, (*) - corresponding WDCM numbers
Storage and Shelf Life
Store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Detection Kit- Mucormycosis |
|---|---|
| SKU | K144 |
| Customized Product Available | No |
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