HiCulture™ Transport Swabs w/ Amies Medium w/o Charcoal with metal stick

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SKU:
MS684S


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Directions

Using the capped swab, provided along with the media containing tube, collect the sample to be transported.Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will diminish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The cultures on transport swabs must not be kept at room temperature formore than 24 hours.

Principle And Interpretation

Transport medium is necessarily and should be non-nutrient, semisolid, reductive medium which hampers the self destructive enzymatic reactions actions within the cells and also inhibits toxic oxidation and also inhibits toxic oxidation effects. Transport Medium, Amies without Charcoal was formulated by Amies (1) by modifying Cary and Blair's medium (2) for transporting microbiological specimens. Amies transport medium is a modification of Stuarts Transport Medium, in which glycerophosphate is replaced by inorganic phosphate buffer. This prevents problem of overgrowth of contaminating organisms while carrying faecal specimens containing Shigella which derive energy from glycerophosphate. Calcium and magnesium salts control the permeability of bacterial cells (1). Presence of sodium thioglycollate and small a mounts of agar provide a reduced environment (3). Sterile cotton swabs allow absorption of specimen material while metal shaft allows semiflexibility to the swab stick, aiding in collection.

Quality Control

Appearance: Sterile Amies medium without charcoal in tubes with metal stick.

Colour: Colourless medium

Quantity of Medium: 8ml of medium in tubes

Reaction: 7.00-7.40

Cultural response

Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35 - 37°C for 18-24 hours.

Organism Recovery
Neisseria meningitidis ATCC 13090 Luxuriant
Staphylococcus epidermidis ATCC 12228 Luxuriant
Staphylococcus aureus ATCC 25922 Luxuriant
Streptococcus pyogenes ATCC 19615 Luxuriant

Sterility test: Passes release criteria

Storage and Shelf Life

Store between 5 – 25°C with caps firmly screwed. DO NOT FREEZE. Use before expiry date on label.

More Information
Product Name HiCulture™ Transport Swabs w/ Amies Medium w/o Charcoal with metal stick
SKU MS684S
Customized Product Available No
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