HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Prepared Transport Medium with Swabs#4fa25a
Intended Use
Recommended for recovery of aerobic, anaerobic and fastidious bacteria from throat, vaginal and wound specimens.
Directions
Using the capped swab, provided along with the media containing tube, collect the sample to be transported.Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will diminish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The cultures on transport swabs must not be kept at room temperature for more than 24 hours.
Principle And Interpretation
Effective recovery of microorganisms and its identification is dependenton a number of factors such as collection and transportation to the laboratory under conditions which allow maintenance of viability. Amies transport medium w/ charcoal is a modification of Stuarts Transport Medium (1,2,3,4). The medium gives improved recovery of Neisseria gonorrhoeae due to presence of charcoal in the medium. Charcoal neutralizes the toxic effects of material that may hamper growth of pathogens. Addition of inorganic phosphate buffer and sodium chloride make this medium different from stuarts transport medium. Calcium and magnesium salts control the permeability of bacterial cells. Presence of sodium thioglycollate and small amounts of agar provide a reduced environment. Sterile cotton swabs allow absorption of specimen material while metal shaft allows semiflexibility to the swab stick, aiding in collection.
Quality Control
Appearance
Sterile Amies medium with charcoal in tubes with metal stick.
Colour
Black coloured medium
Quantity of Medium
8ml of medium in tubes
pH
7.00-7.40
Sterility test
Passes release criteria
Cultural response
Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35 - 37°C for 18-24 hours.
| Organism | Recovery |
|---|---|
| Pseudomonas aeruginosa ATCC 27853 | Luxuriant |
| Escherichia coli ATCC 25922 | Luxuriant |
| Klebsiella pneumoniae ATCC 13883 | Luxuriant |
| Neisseria meningitidis ATCC 13090 | Luxuriant |
| S. serotype Typhi ATCC 6539 | Luxuriant |
| Shigella flexneri ATCC 12022 | Luxuriant |
| Staphylococcus aureus ATCC 25922 | Luxuriant |
Storage and Shelf Life
Store between 5 – 25°C with caps firmly screwed. DO NOT FREEZE. Use before expiry date on label.
| Product Name | HiCulture™ Transport Swabs w/ Amies Medium w/ Charcoal with metal stick |
|---|---|
| SKU | MS651S |
| Customized Product Available | No |
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