HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Yeast Extract Rose Bengal HiVeg™ Broth Base
For the cold enrichment for the recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from food samples as per APHA.
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Animal Free Culture Media (HiVeg™)#4fa25a
Composition
| Ingredients | Grams/Litre |
|---|---|
| Yeast extract | 5.0 |
| Disodium phosphate | 17.25 |
| Synthetic detergent | 2.0 |
| Sodium chloride | 1.0 |
| Magnesium sulphate | 0.01 |
| Sodium pyruvate | 1.0 |
| Rose Bengal | 0.04 |
Final pH (at 25°C) 7.9 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 26.3 grams in 900 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 20-25°C and aseptically add 100 ml of 4% filter sterilized sorbose solution. Mix well and dispense aseptically as desired.
Principle and Interpretation
This medium is prepared by using synthetic detergent in place of bile salts that make the medium free of BSE/TSE risks. Yeast Extract Rose Bengal HiVeg Broth Base is the modification of Yeast Extract Rose Bengal Broth Base which is formulated as recommended by APHA for enrichment of Yersinia species from foods (1). Yeast extract provides essential nutrients. Synthetic detergent inhibit gram-positive organisms. Salts help in cold enrichment of Yersinia species.
Yersinia enterocolitica and Yersinia pseudotuberculosis can grow at 4°C, so primary enrichment is carried out in this medium for 9 days at 4°C or 3 days at 10°C (2). From this enrichment medium the organisms are further enriched in secondary selective enrichment medium such as PSTA HiVeg Broth (MV940) and then isolated by streaking onto selective plating media such as Yersinia Selective HiVeg Agar (MV843), SS HiVeg Agar (MV108) and MacConkey HiVeg Agar (MV082).
Quality Control
Appearance of powder
Pink coloured, homogeneous, free flowing powder.
Colour and Clarity
Reddish pink coloured, clear solution without any precipitate.
Reaction
Reaction of the medium (2.63% w/v Base + 0.4% w/v sorbose in distilled water) is pH 7.9 ± 0.2 at 25°C.
Technical Data
Product Profile :
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV955 | M955 |
| Synthetic detergent | Bile salts |
Recommended for : Cold enrichment in the recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods.
Reconstitution : 26.30 g/l
Quantity on preparation (500g) : 19.01 L
pH (25°C) : 7.9 ± 0.2
Supplement : 4% filter-sterilized sorbose solution
Sterilization : 121°C / 15 minutes.
Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Cultural Response
Cultural characteristics observed after an incubation at 4°C for 9-10 days.
| Organisms (ATCC) | Recovery |
|---|---|
| Yersinia enterocolitica (27729) | good-luxuriant |
| Yersinia pseudotuberculosis (29833) | good-luxuriant |
References
- Downs F.P. and Ito K (Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 4th ed., APHA, Washington, D.C.
- Schiemann D.A., 1982, Appl. Environ. Microbiol., 43:14.
| Product Name | Yeast Extract Rose Bengal HiVeg™ Broth Base |
|---|---|
| SKU | MV955 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Cover T. L. and Aber R. C., 1989, N. Engl. J. Med. 321:16-24 |
| Customized Product Available | No |
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