HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
Your enquiry has been submitted
Something problem with the server. Try again.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone, special | 5.000 |
| Yeast extract | 5.000 |
| Dextrose | 10.000 |
| Monopotassium phosphate | 0.500 |
| Potassium chloride | 0.125 |
| Calcium chloride | 0.125 |
| Sodium chloride | 0.125 |
| Magnesium sulphate | 0.125 |
| Manganese sulphate | 0.003 |
| Bromo cresol green | 0.030 |
| Tomato juice solids, from | 150.000 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
The test sample should be filtered through a sterile membrane filter having pore size of 0.45µ. Rehydrate the nutrient pad with 2.0 -2.5 ml sterile distilled / purified water. After filtration, remove the membrane filter aseptically using sterile forceps. Place the membrane filter on rehydrated nutrient pad. Incubate the inoculated nutrient. Interpret the results qualitatively by observing the presence or absence of growth and quantitatively by counting the number of colonies on the surface of the membrane filter and calculating CFU/ml.
Principle And Interpretation
Field of Application: Beverages. DriFilter Membrane Nutrient Pad Medium is ready to use sterile culture media in the form of a 50 mm biological inert absorbent pads impregnated with Tomato Juice medium, then dried and sterilized in 55 mm petri plate. They eliminate the need of laborious media preparation and autoclaving procedures. The nutrient pads are to be just rewetted with sterile distilled water and are ready to use. Use of nutrient pads allows larger sample volumes to be tested at a time. Interpretation of results is directly by counting the CFUs and also quantifies the microbial load present in the sample. Mickle and Breed (1) first described the use of tomato juice in the culture media for Lactobacilli. Tomato Juice Medium is recommended for the cultivation of yeast and other aciduric organisms (2) and is based on the formula of Kulp and White for cultivation of yeasts and other aciduric microorganisms (3). Ability of tomato juice to enhance the recovery of Lactobacilli was observed by Kulp (4). Tomato juice acts as a source of carbon, nutrients and proteins. Yeast extract provides nitrogenous compounds and amino acids which stimulate the growth of spoilage strains (5). Low pH of the medium encourages growth of Lactobacilli while inhibiting the growth of accompanying bacteria. Phosphates buffer the medium. Magnesium sulphate, manganese sulphate and ferrous sulphate provide inorganic ions. Sodium chloride maintains osmotic balance in the medium. Field of Application:
Quality Control
Appearance
Dry filter membrane pad of 50mm diameterDry filter membrane pad of 50mm diameter
Colour
Pale coloured nutrient pad
Sterility test
Passes release criteria
Cultural response
Cultural characteristics observed after incubation at 35-37°C for 48-72 hours
| Organism | Growth | Colour of colony |
|---|---|---|
| Lactobacillus casei ATCC 9595 | Luxuriant | Colourless |
| Lactobacillus leichmannii ATCC 4797 | Luxuriant | Colourless |
Storage and Shelf Life
Store between 10-30°C. Use before expiry date on the label.
| Product Name | Tomato Juice Medium w/ Sterile Membrane filter |
|---|---|
| SKU | MF021F |
| Customized Product Available | No |
Write Your Own Review